Staining
What is the name of the primary stain
Crystal Violet
Gram positive cocci, catalase positive, coagulase positive
S. aureus
Gram negative rod, strong lactose fermenter, metallic sheen on EMB.
E. coli
Most non-fermenters are ____ positive?
Oxidase
What media is used to screen for gram negatives
MAC/EMB
What acts as a mordant
Iodine
Gram positive cocci, catalase negative, beta hemolytic, bacitracin susceptible
S. pyogenes
Possesses a capsule, lactose fermenter, citrate positive
Klebsiella species
What biochemical is often used to tell the difference between K.pneumoniae and K. oxytoca?
Indole
What agar is used in Cystic Fibrosis sputum cultures to isolate Burkholderia cepacia?
BCSA
Reagent used as decolorizer
Acetone/Alcohol
Gram positive cocci, catalase negative, narrow zone of hemolysis, CAMP positive
S. agalactiae
Swarming motility, indole positive
Proteus vulgaris
Gram positive cocci, ferments bile esculin
(think back to our lab with the BEA plate)
Enterococcus species
What plate is used to screen for E.coli 0157: H7?
SMAC
Color that WBC's should appear
Pink
Gram positive cocci in lancet shapes, mucoid alpha hemolytic colonies, bile soluble, and optochin sensitive
S. pneumoniae
Oxidase positive Enterobacterales stool pathogen
Plesiomonas species
Oxidase negative, biochemically inert, associated with ventilator nosocomial infections, difficult to treat
A. baumanii
What plate is used to screen for Yersenia, which is kept at 23 degree celsius?
CIN
Why won't mycoplasma stain with a gram stain?
They lack a cell wall
Second most common cause of UTI's in sexually active females, routinely susceptible to nitrofurantoin
S. saprophyticus
H2S production on HE/XLD, stool pathogen
Salmonella species
PAD positive, NLF
Proteus, Morganella, Providencia
What media is used for the isolation of Vibrio, which causes rice water stools?
TCBS