What is the long instrument with a loop at the end called?
An inoculator
100
What kingdom of pathogens is prokaryotic?
Eubacteria
100
What color do gram positive bacteria stain?
Purple
100
What are 3 shapes of bacteria?
Rod (bacillus), round (coccus), and spiral (spirilla or spirochete)
200
Where do sharp objects and used slides get disposed of in the lab?
Sharps container
200
Where are bacteria grown in our lab?
Incubator
200
What pathogen kingdom has peptidoglycan cell walls?
Eubacteria
200
How is gram positive different from gram negative in terms of cell wall?
Gram + has a thick cell wall and gram- has a thin cell wall.
200
How do differential and selective nutrient plates differ?
Differential compares and contrast 2 growing colonies.
Selective suppresses one type and grows another.
300
What 3 essential small pieces of safety equipment should be mounted and in view in the lab?
Fire extinguisher, eye wash and first aid kit
300
What piece of equipment sterilizes test tubes and other glassware in the lab?
Autoclave
300
What two pathogen kingdoms are microscopic?
Eubacteria and Protista
300
What are three common gram positive bacteria?
Mouth plaque, Strep, and Staph
300
Explain two ways that microbes can get energy.
Cellular respiration (aerobic)- 36 ATP
Fermentation:2 ATP
400
How should a microbiology spill be handled?
Shout "We have a spill" Don't move. "Is everybody Ok? Spray the area with disinfectant and the lay down paper towels for 15 mins. Discard all papers in Biohazard.
400
Why are close-toed shoes used in the microbiology lab?
To avoid injury and contamination if glass should break or a vial should leak.
400
What pathogen kingdoms can be either autotrophic or heterotrophic?
Eubacteria and Protista
400
Name 3 gram negative bacteria.
Bordetella pertussis
Yersinia pestis
Salmonella
400
What are four types of bacterial requirements?
Nutrients, time, temperature and atmosphere.
500
What do aseptic techniques in the OR do?
Separate and guard sterile and nonsterile areas
500
What general rule should you observe when handling microbes?
All microbes should be considered dangerous.
500
How are Animals different from Fungi or Plantae?
Kingdom Animalia has cells with no cell walls.
500
What is the gram stain procedure?
Use a heat dryed bacterial sample on a slide
Crystal Violet 1 min and rinse
Iodine 1 min and rinse
Acetone rinse
Safranin 1 min and rinse
500
How are microbes identified?
Using a flowchart or dichotomous key of microscope results and chemical tests.