Media
Enterobacteiaceae
Gram Stain
Types of bacteria
Laboratory policies
100
Select the primary and differential media of choice for recovery of most fecal pathogens.
What is Hektoen, MacConkey, MacConkey-Sorbitol, Campy blood, colistin-nalidixic acid (CNA) agar, Selenite-F broth
100
Biochemically, Enterobacteriaceae species are gram-negative rods that:
What is ferments glucose, reduce nitrate to nitrite, and are oxidase negative
100
What are the two most common bacterial shapes?
What is rod and cocci
100
This type bacteria is able to live in the colon with little to no oxygen and is the predominant organism
What is anaerobes
100
Quality control is designed to
What is to ensure the medical reliability of the laboratory data/results
200
CNA agar is used primarily for the recovery of:
What is Gram positive organisms
200
The Voges-Proskauer (VP) test detects which end product of glucose fermentation?
What is Acetoin or cabinol
200
What is this gram stain result?
What is Streptococci: gram-positive cocci in long chains
200
Aerobes are bacteria that grow
What is ambient air
200
Why should health care workers wash their hands after coming in contact with a patient?
What is to reduce the spread of pathogenic bacteria from one individual to another
300
Cycloserine-cefoxin-fructose agar (CCFA) is used for the recovery of:
What is Clostridium difficile
300
Which of the following tests best differentiates Shigella species from E. coli?
What is lactose, indole, ONPG, and motility
300
This constituent of a gram-positive cell wall absorbs crystal violet but is not dissolved by alcohol, thus giving the gram-positive cell its characteristic purple color.
What is Peptidoglycan
300
Microaerophiles are bacteria that grow
What is in reduced oxygen concentration by increased carbon dioxide concentrations
300
Gloves, laboratory coats, safety glasses are all examples of:
What is PPE
400
What is the medium best suited for the recovery of Y. enterocolitica from a patient with gastroenteritis?
Cefsulodin-irgasan-novobiocin (CIN) agar
400
Which single test best separates Klebsiella oxytoca from Klebsiella pneumoniae?
What is indole
oxytoca is positive and pneumoniae is negative
400
The purpose of a simple stain is to
What is color forms (positive/negative) and shapes (cocci/rods) present.
400
Capnophiles are bacteria that grow
What is increased carbon dioxide concentrations
400
The chemical or physical method that destroys all forms of life is
What is sterilization
500
The purpose of transport media is to
What is to ensure the preservation of microorganisms in the specimen
500
The drugs of choice for treatment of infections with Enterobacteriaceae are:
What is aminoglycosides, sulfamethoxazole-trimethoprim, third-generation cephalosporins
500
What stain is used for medically important fungi?
What is lactophenol cotton blue
500
Health people are colonized by many different bacteria in many different sites. These bacteria are referred to as
What is indigenous flora (normal flora)
500
Universal/standard precautions require that
What is blood and body fluids from all patients be considered infectious and capable of transmitting disease