Paper: Methods
What was the total n of this study?
What is 54,198
True or false: L. asaccharolyticus is not present in people who never drink coffee.
Coffee beans are not actually beans, they are:
What are fruit seed/pits
What % of all disease can some way be traced back to the gut and health of the gut microbiome?
What is 90%
Overgrowth of this bacterial species can occur after antibiotic use, leading to infection/inflammation.
What is Clostridium dificile
What are the types of coffee that were tested for ability to stimulate L.asaccharolyticus growth in vitro?
What are: 1) brewed, 2) brewed decaffeinated, 3) instant, and 4) instant decaffeinated.
How many ancient human microbiome samples were tested? How many had A.saccharolyticus present?
What is: 37 and 2.
What country is the largest producer of coffee widely known as the coffee powerhouse?
What is Brazil (produces 40% of the world's coffee!)
True or false: Humans differ more from each other via their microbial genes than via their human genes?
What is true.
What is the number of non-redundant genes present in the human microbiome?
What is 3.3 million
What are E.coli and B.fragilis.
Which type of coffee stimulated L. saccharolyticus growth on agar plates the most at both the 5 g/L and 10 g/L doses?
True or false: decaffeinated coffee does not contain caffeine
What is false
What are the six main phyla of bacteria present in the gut microbiome?
What are 1) Firmicutes, 2) Bacteriodetes, 3) Actinobacteria, 4) Proteobacteria, 5) Verrucomicrobia, and 6) Fusobacteria.
How many vitamins are exclusively produced by microorganisms?
Just one! Vitamin B12. Animals, plants and fungi are incapable of production and it is the only vitamin that is exclusively produced by microorganisms, particularly by anaerobes.
What does PREDICT stand for?
Personalized Responses to Dietary Consumption Trial
Name 3 metabolites of coffee that were correlated with coffee intake.
What are: caffeine, 1-methyluric acid, 1,7-dimethyluric acid, 1-methylxanthine, 3-methylxanthine and 5-acetylamino-6-amino-3-methyluracil, quinic acid, and trigonelline
What famous composer was very specific about loving coffee, theorizing that "the perfect cup of coffee must be made from 60 coffee beans; no more, no less"
What is Beethoven
What mucus-degrading bacterial species has recently been linked to multiple sclerosis?
What is Akkermansia muciniphila
Which bacterial species produces polysaccharide A?
What is Bacteroides fragilis
What cohort was used for the metabolomic studies?
the MLVS cohort
Which country had the highest coffee consumption per capita?
What is Luxembourg
What is 15
What are the two organs of the body in which the presence of a microbiome has been hotly debated?
What are the placenta and the brain
What % of the body's serotonin is produced in the gut?
What is 90-95%