Definitions
the quantity of light absorbed by a solution
What is Absorbance?
What is the crucial first step in DNA Extraction and Purification?
What is Extraction?
What is Obtaining DNA?
The ideal final quadrant should show this type of colony distribution
What are isolated, single colonies?
This is the primary purpose of performing a serial dilution in a laboratory setting?
What is reducing bacterial concentration?
This is the primary purpose of Gram staining in microbiology. Include their visual indicator.
What is differentiating bacteria into Gram-positive (purple) and Gram-negative (pink)?
the amount of a substance that is in a certain amount of tissue or liquid. ie "How much ______ is present in this sample?"
What is Concentration?
What is the common purification method used after extraction?
What is Centrifugation?
The type of medium that is used for Quadrant Streaking
What is solid agar?
This is the type of medium used in serial dilution?
Typically a stock solution of bacteria is used.
The main purpose of simple staining.
What is visualizing bacterial cells under a microscope?
A common process used for DNA purification that separates particles from a solution according to their size, shape and density
What is Centrifugation?
What is the purpose of DNA Extraction and Purification?
What is Separating DNA from other unwanted components of the cell (RNA and proteins)?
This is why the plate is always incubated upside-down.
What is preventing condensation from dripping onto the agar?
This is the reason why we want to reduce bacterial concentration.
What is reducing bacterial concentration to a countable level?
The main difference between simple staining and differential (gram) staining.
What is simple staining uses one dye, while differential staining uses multiple?
the collection of procedures and techniques designed to prevent the introduction of unwanted organisms into a pure culture
What is Aseptic Technique?
What is a visual indicator that DNA extraction and purification was successful?
What is The presence of a stringy white mass in solution?
What is the primary purpose of Quadrant Streaking?
What is isolation of individual bacterial colonies from a mixed sample.
In a 1:10 dilution, 1 mL of sample is added to this amount of diluent.
What is 9ml?
The reason Gram-positive bacteria retain the primary stain.
What is thick Peptidoglycan layer?
the separation of a strain from a mixed population of living microbes
What is Bacterial Isolation?
the process of breaking down the cell membrane through physical, chemical, or enzymatic methods, allowing the release of intracellular components such as proteins, nucleic acids, and small molecules
What is Cell Lysis?
If colonies grow throughout all four quadrants without isolation, this could be the mistake.
What is failing to sterilize the loop between streaks? (Not using aseptic technique)
This is how I calculate Dilution Factor.
What is Volume of Stock Solution / (Volume of Stock Solution + Volume in Tube)
The critical step that differentiates Gram-positive from Gram-negative bacteria.
What is decolorization with alcohol or acetone?