Methods
Quality
Enzyme & Immune
Automation/POCT
Hodge Podge
100
Potentiometry
What is ion selective electrodes measure potential difference across a membrane.
100
The degree to which a test or study measures what it purports to measure.
What is validity
100
Enzyme
What is biological catalyst that decreases the activation energy of a chemical reaction?
100
Dry slide system
What is reagents are impregnated on films of filter paper strips of the slide?
100
24 hour biological clock
What is circadian variation?
200
A highly sensitive and specific chromatographic method that uses an injector to send the mobile phase into the column.
What is HPLC?
200
CLSI recommendation for RCF.
What is 1200g for 10 minutes?
200
First order kinetics vs. zero order kinetics
What is amount of substrate present in proportion to enzyme vs. rate of reaction is independent of reactant concentration?
200
Two types of LIS
What are unidirectional and bidirectional?
200
Steps in PCR
What is denature, anneal, extend?
300
Beer's Law
What is absorbance is directly proportional to concentration?
300
Effect of hemolysis on LD and potassium
What is both will increase?
300
Heterogenous, non-competitive immunoassay
What is ELISA?
300
Advantages of POCT
What are reduced TAT, improvement of health outcomes, lower cost
300
Protein associated with emphysema
What is alpha 1 antitrypsin?
400
The unfragmented ion of the original molecule being studied in mass spectrometry.
What is a molecular ion?
400
Systemic error vs. random error
What is problem with instrument vs. problem with specimen
400
Hapten
What is a substance that cannot elicit an immune response alone, but must be conjugated to a carrier molecule to cause antibody production?
400
Diagnosis of DM
What is fasting glucose over 126 mg/dl, Two hour GTT or random glucose >200mg/dl
400
The most abundant protein in plasma that transports and is a negative APR
What is albumin?
500
In a mass spec, this keeps the ions from colliding.
What is the vacuum system?
500
True negatives/(false positives + true negatives) x 100
What is clinical specificity?
500
Transfer of electrophoresed protein from a gel onto a solid support to fix proteins in place for further immune-processing.
What is Western blot?
500
Cause of Type II DM
What is resistance to action of insulin and beta cell dysfunction?
500
Significance of colors in NFPA hazard diamond.
What is red=flammability, blue=health, yellow=reactivity, white=other
M
e
n
u