Morphology and Hematopoeisis
What is the main function of bone marrow?
To maintain a steady supply of mature hematopoietic cells to blood circulation.
What is the normal life span of RBCs in circulation?
120 days
How many cells are counted in a MANUAL retic count?
1000
Approximately what percentage of RBCs are removed daily from a healthy adult?
1%
What is the difference in accuracy and precision?
Accuracy: adherence to true value
Precision: Reproducibility
What is meant by medullary hematopoiesis?
Hematopoiesis within the bone marrow
Which electrolyte is highest INSIDE the RBC?
Potassium (K+)
What cellular remnants in a reticulocyte are visible when stained with new methylene blue?
RNA
What are the reference rages for Hemoglobin and Hematocrit?
Hgb: 12-18%
Hct: 35-50%
If a standard deviation has been calculated as 5 with a mean of 25, what would the reference range be?
20-30
What is the site of Erythropoietin production?
Kidney
RBC membrane proteins that are limited to the cytoplasmic surface of the membrane are called what?
Peripheral proteins
A patient whose RBC indices are MCV = 80 fL, MCH= 28 pg, and MCHC= 34% would be classified as:
Normocytic/Normochromic
Codocyte is another name for which type of red cell?
Target cell
Automated CBC instruments use the principle of what?
Electrical Impedance
Normal reference range for Neutrophils, Lymphocytes, and Monocytes.
Neutrophil: 50-70%
Lymphocyte: 18-42%
Monocyte: 2-11%
What does normal adult hemoglobin A consist of?
2 alpha chains
2 beta chains
4 heme groups
Heinz bodies may be seen in what hematologic disease?
G6PD deficiency
Define the following acronyms and give a definition of the word:
MCV
MCH
MCHC
MCV: Mean Corpuscular Volume; Used to estimate the average size of the RBC
MCH: Mean Corpuscular Hemoglobin; average mass of hemoglobin per red blood cell in a sample of
blood
MCHC: Mean Corpuscular Hemoglobin Concentration; measure of the concentration of hemoglobin in a given volume of
packed red blood cells
Through what principle do automated instruments measure cell size and cell density?
Cell size-Forward light scatter
Cell density-side light scatter
What is the most appropriate site for a bone marrow aspiration in adults?
The iliac crest
This abnormal hemoglobin forms when iron is oxidized to the ferric (Fe3+) state and normally less than 1% of the total hemoglobin is in this state in a healthy individual.
Methemoglobin
A normal size red cell with a slit-like shaped central pallor is called a:
Stomatocyte
What are the three classifications of anemias?
Normocytic/Normochromic
Microcytic/Hypochromic
Macrocytic/Normochromic
WBC/µL = Avg # cells counted x 100
# squares counted x 0.1/µL
Number of cells counted in side 1 = 27
Number of cells counted in side 2 = 29
Average Number = 28
WBC/µL = 28 x 100 = 7.0 x 103/µL
4 x 0.1