amount of absorbance of a solution is directly proportional to the concentration of that solution
Basic principle of Spectrophotometry, Beer's Law
Ability to obtain quality control results that are close to the true value
accuracy
In electrophoresis, the pH at which the number of positively charged ions and the number of negatively charged ions balance each other
Isoelectric point
The most common medium for electrophoresis
agarose gel
To obtain the most accurate result, one would choose the wavelength which shows:
maximum absorbance
A Guassian distribution is usually this shape
Bell
same test is performed on all samples before next test is performed
Batch testing
Release of light energy as an end product of a chemical reaction
chemilluminescence
The component of the spectrophotometer responsible for the isolation of the wavelength is the:
Monochromator
An example of an external quality control program
Proficiency Testing
A substance used in the laboratory that contains an exact amount
standard
Measurement of light scatter from an angle
Nephelometry
using the spectrophotometric technique, what range is the visible spectrum
380-740
Q.C. data shows an increase # in one of the tails of distribution
Skewness
In this type of automation, analyses are performed on a collection of specimens sequentially and each specimen is analyzed for a different selection of tests.
random access
methodology which works by measuring the electrical potential difference between the reference solution and the unknown test solution
ISE
Type of automation that includes thin-layer, gas, and high performance.
Chromatography
The reliability of a test being negative when a condition to be detected is not present
diagnostic specificity
Procedures that rely on the use of antibodies as specific binding reagents
Immunoassays
light source is used in atomic adsorption spectrophotometry
hollow cathode lamp