DNA
These structures protect the ends of the chromosome from a loss of information during replication
Telomeres
Replication of DNA is in this direction
5' to 3'
This anticoagulant is a no-no for samples sent for molecular testing
Heparin
SYBR Green is ggggreat because it only binds to....
DS DNA! (new product)
A northern blot detects
RNA targets
Name the two pyrimidine bases
Cytosine
Thymine
A silent mutation often is silent because it occurred in this position within the codon
wobble position
I say aneuploidy of daughter cells following meiosis,
you say....
Nondisjunction
Melt-curve analysis is done after this type of PCR reaction, to ensure you got the right product
Real-Time PCR
The spectrophotometric absorbance for DNA/RNA is ______ and protein is ________
260/280
Match the deoxyribose carbon number to its attached group.
C1, C2, C3,C5 (Carbons)
Nitrogen base, OH, Hydrogen, Phosphate (Groups)
C1-Nitrogen Base
C2-Hydrogen
C3- OH
C5- Phosphate
This enzyme brings amino acids to the site of translation and matches each amino acid to a specific mRNA codon
transfer RNA
Cut DNA at specific, often palindromic, sequences
Are thought to be a defense mechanism bacteria use against bacteria-infecting viruses
Recognize sequences that are not in the genome of the bacteria from which they originate
Describes what enzyme?
Restriction endonuclease
The cycle number at which a sample reaches a preset level of fluorescence is called the ____________
This methodology uses fluorescently labeled probes to detect DNA, RNA, or proteins inside a cell
FISH
This is the difference between a RIBOSE sugar and a DEOXYRIBOSE sugar
Ribose sugar has -OH on both the 2' and 3' Carbon Deoxyribose sugar has -OH on only the 3' carbon
This life-saver repairs the sugar phosphate backbone of the lagging strand during replication
ligase
In the UNG system used to prevent amplicon contamination in the MDx lab, I would need to make this change to my reagents for the process to be effective...
use dUTP's instead of dTTP's
Another name for an RNA dependent DNA Polymerase is....
Reverse Transcriptase
Monitoring Hep C viral load in a patient blood sample would best be accomplished using which method?
qRTpcr
This is where DNA methylation usually occurs in the genome
CpG islands in a coding region of a gene
A poly AAA tail and a guanine "cap" with spliced out introns describes this important molecule (it has U's in it too)...
mRNA (messenger)
The temperature used in the annealing step of PCR is determined by
GC content/length of the primer
The complimentary sequence to this strand of DNA is (tell me from the 3' end....):
5'-ATC CCG TTG AAA CTC GGT-3'
3'-TAG GGC AAC TTT GAG CCA-5'
Double jeopardy! Make a wager
Name 5 ingredients needed for PCR
Name one additional ingredient needed for sanger sequencing