2D Electrophoresis
This parameter serves as the first separation dimension during 2D electrophoresis.
What is isoelectric point?
An early step of Western blotting involves separating the proteins present in a mixture based on their size, using this technique.
What is SDS PAGE gel electrophoresis?
These short sequences of DNA target unique sequences of the genome, making PCR highly specific.
What are primers?
This method is performed to linearize plasmids so that they can be visualized in a gel as various sized pieces.
What is restriction digestion?
This is the equation you should apply if you need to dilute a strong stock solution into a working solution.
What is C1V1=C2V2?
BONUS These are Jessy's pets.
What are a dog and a cat?
The use of this in Western blotting underlies it's specificity at targeting a single protein.
What is an antibody?
This type of PCR uses the starting product of RNA, rather than DNA.
What is reverse transcription PCR?
This attribute was incorporated into the plasmid in order to select for bacterial cells that had been succesfully transformed.
What is AMP resistance?
This is the equation you need to interpolate unknown data from a standard curve.
What is y=mx+b?
These are the two functions of SDS during SDS-PAGE.
What are linearization of the protein, and applying a charge to the proteins?
BONUS
This classmate is also a director, writer, and singer
Who is Rojin?
These tests are used to determine the specificity of a PCR reaction (hint: there are three)
What are gel electrophoresis, melt curve analysis, and primer efficiency
These two techniques were used to deliver plasmids to cells.
What are electroporation (bacteria) and lipofectamine (mammalian cells)?
In PCR, this is how you refer to the cycle number at which the machine detected SYBR green signal.
What is Ct value?
Clean-up is necessary in 2D electrophoresis in order to remove this, which would otherwise interfere with the process.
What is lysis buffer
Skipping this step may lead to a poor signal-to-noise ratio, also referred to as high background.
What is blocking non-specific binding?
This is the critical temperature that ensures optimal binding of primers to denatured DNA strands, in order to amplify the gene of interest every cycle.
What is the annealing temperature?
This attribute was included in the plasmid to allow identification of whether a mammalian cell had undergone transfection.
What is GFP?
BONUS
These are Crystal's children's names
What are
Lilian, Emily, Audrey, Maggie
This additional resolution obtained during 2D electrophoresis make it a superior choice to single dimension options like SDS-PAGE.
What is resolution based on isoelectric point.
Sample dilution occurred differently when performing the BCA assay vs the SDS PAGE, due to this phenomenon.
What is buffer interference?
Without the information about this gene's expression, it is impossible to calculate relative expression of a gene of interest.
What is a housekeeping gene?
These enzymes allow for size-based identification of plasmids.
What are restriction digestion enzymes?
In an experiment where you manipulated one variable across four different groups, this statistical test can be used to determine differences between groups.
What is a One Way ANOVA?