VNTR/STR
Back to the Basics
Who's Your Daddy?
What Your Momma Gave You!
Everything Else

100

Repetitive sequences of typically 8 to 80 base pairs
What is VNTR

100

What is one use of forensic analysis?
identify a person identify a clinical specimen organ donor determination parental testing

100

What is the most common method of parental testing?
STR analysis

100

True or False: Mitochondrial DNA degrades faster than chromosomal DNA.
False

100

What is the name of the FBI database for STR analysis?
CODIS

200

True or false: VNTR was the favored method of analysis up until the 1990's?
True!

200

Name two samples that can be used for testing.
Blood Buccal mucosa cellular remains hair bone marrow paraffin-embedded tissues

200

True or false: A child inherits both haplotypes of the mother.
False. One from the mother and one from the father

200

What is the name of the 1200 bases that are analyzed in mitochondrial DNA testing?
Displacement loop (D-Loop)

200

What is the name of the PCR technique used most commonly in forensic laboratories?
Capillary electrophoresis

300

The repetitive sequence of 2 to 7 base pairs.
What is STR

300

What is forensic testing designed to detect?
polymorphisms

300

What is one requirement for the sample of parentage testing?
Must contain chromosomal DNA

300

Name a sample that requires mitochondrial DNA testing.
Hair with no roots old skeletal remains

300

What is the low molecular weight protein that can be analyzed to determine the sex of the individual?
Amelogenin

400

What are 2 reasons VNTR is not preferred for forensic analysis?
Disadvantages: expensive labor intensive difficult to automate less sensitive DNA degradation Requires a larger sample

400

What other 2 things can you test besides cellular DNA?
Sex chromosomes Mitochondria

400

Ms. Anderson unexpectedly gives birth and falls asleep shortly after. Her husband accidently puts their baby down in the wrong bassinet and now they don't know which baby is theirs! What type of testing can they do to identify their baby?
Reverse Parentage Testing

400

Why does mitochondrial DNA stay constant through generations (except for mutation)?
It doesn't undergo meiosis

400

Name 2 pre-analytical issues that can occur in crime scene analysis.
contamination degradation wrong collection tubes coagulated blood

500

What is the favored type of analysis and give 2 reasons why.
STR because: small samples quick less expensive Takes less training Less sensitive to degradation can be automated

500

Everyone's DNA is unique in every case except?
In Identical Twins.

500

Child HLA Type: A1, A92, B7, B41, C12, C14 Mother HLA Type: A14, A92, B7, B7, C14, C22 Who is the Father? Possible Fathers: 1st HLA Type: A1, A13, B8, B18, C33, C54 2nd HLA Type: A1, A1, B7, B44, C22, C66 3rd HLA Type: A1, A34, B41, B41, C12, C66
3

500

Why do you analyze the D-loop of the mitochondrial DNA?
Because it contains 2 hypervariable regions that are useful for ID testing

500

What is one clinical use for DNA analysis?
ID a specimen Prenatal testing organ transplants