VNTR/STR Back to the Basics Who's Your Daddy? What Your Momma Gave You! Everything Else

100

What is VNTR
Repetitive sequences of typically 8 to 80 base pairs

100

identify a person identify a clinical specimen organ donor determination parental testing
What is one use of forensic analysis?

100

STR analysis
What is the most common method of parental testing?

100

False
True or False: Mitochondrial DNA degrades faster than chromosomal DNA.

100

CODIS
What is the name of the FBI database for STR analysis?

200

True!
True or false: VNTR was the favored method of analysis up until the 1990's?

200

Blood Buccal mucosa cellular remains hair bone marrow paraffin-embedded tissues
Name two samples that can be used for testing.

200

False. One from the mother and one from the father
True or false: A child inherits both haplotypes of the mother.

200

Displacement loop (D-Loop)
What is the name of the 1200 bases that are analyzed in mitochondrial DNA testing?

200

Capillary electrophoresis
What is the name of the PCR technique used most commonly in forensic laboratories?

300

What is STR
The repetitive sequence of 2 to 7 base pairs.

300

polymorphisms
What is forensic testing designed to detect?

300

Must contain chromosomal DNA
What is one requirement for the sample of parentage testing?

300

Hair with no roots old skeletal remains
Name a sample that requires mitochondrial DNA testing.

300

Amelogenin
What is the low molecular weight protein that can be analyzed to determine the sex of the individual?

400

Disadvantages: expensive labor intensive difficult to automate less sensitive DNA degradation Requires a larger sample
What are 2 reasons VNTR is not preferred for forensic analysis?

400

Sex chromosomes Mitochondria
What other 2 things can you test besides cellular DNA?

400

Reverse Parentage Testing
Ms. Anderson unexpectedly gives birth and falls asleep shortly after. Her husband accidently puts their baby down in the wrong bassinet and now they don't know which baby is theirs! What type of testing can they do to identify their baby?

400

It doesn't undergo meiosis
Why does mitochondrial DNA stay constant through generations (except for mutation)?

400

contamination degradation wrong collection tubes coagulated blood
Name 2 pre-analytical issues that can occur in crime scene analysis.

500

STR because: small samples quick less expensive Takes less training Less sensitive to degradation can be automated
What is the favored type of analysis and give 2 reasons why.

500

In Identical Twins.
Everyone's DNA is unique in every case except?

500

3
Child HLA Type: A1, A92, B7, B41, C12, C14 Mother HLA Type: A14, A92, B7, B7, C14, C22 Who is the Father? Possible Fathers: 1st HLA Type: A1, A13, B8, B18, C33, C54 2nd HLA Type: A1, A1, B7, B44, C22, C66 3rd HLA Type: A1, A34, B41, B41, C12, C66

500

Because it contains 2 hypervariable regions that are useful for ID testing
Why do you analyze the D-loop of the mitochondrial DNA?

500

ID a specimen Prenatal testing organ transplants
What is one clinical use for DNA analysis?

Molecular Forensics

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