VNTR/STR
Back to the Basics
Who's Your Daddy?
What Your Momma Gave You!
Everything Else
100
Repetitive sequences of typically 8 to 80 base pairs
What is VNTR
100
What is one use of forensic analysis?
identify a person identify a clinical specimen organ donor determination parental testing
100
What is the most common method of parental testing?
STR analysis
100
True or False: Mitochondrial DNA degrades faster than chromosomal DNA.
False
100
What is the name of the FBI database for STR analysis?
CODIS
200
True or false: VNTR was the favored method of analysis up until the 1990's?
True!
200
Name two samples that can be used for testing.
Blood Buccal mucosa cellular remains hair bone marrow paraffin-embedded tissues
200
True or false: A child inherits both haplotypes of the mother.
False. One from the mother and one from the father
200
What is the name of the 1200 bases that are analyzed in mitochondrial DNA testing?
Displacement loop (D-Loop)
200
What is the name of the PCR technique used most commonly in forensic laboratories?
Capillary electrophoresis
300
The repetitive sequence of 2 to 7 base pairs.
What is STR
300
What is forensic testing designed to detect?
polymorphisms
300
What is one requirement for the sample of parentage testing?
Must contain chromosomal DNA
300
Name a sample that requires mitochondrial DNA testing.
Hair with no roots old skeletal remains
300
What is the low molecular weight protein that can be analyzed to determine the sex of the individual?
Amelogenin
400
What are 2 reasons VNTR is not preferred for forensic analysis?
Disadvantages: expensive labor intensive difficult to automate less sensitive DNA degradation Requires a larger sample
400
What other 2 things can you test besides cellular DNA?
Sex chromosomes Mitochondria
400
Ms. Anderson unexpectedly gives birth and falls asleep shortly after. Her husband accidently puts their baby down in the wrong bassinet and now they don't know which baby is theirs! What type of testing can they do to identify their baby?
Reverse Parentage Testing
400
Why does mitochondrial DNA stay constant through generations (except for mutation)?
It doesn't undergo meiosis
400
Name 2 pre-analytical issues that can occur in crime scene analysis.
contamination degradation wrong collection tubes coagulated blood
500
What is the favored type of analysis and give 2 reasons why.
STR because: small samples quick less expensive Takes less training Less sensitive to degradation can be automated
500
Everyone's DNA is unique in every case except?
In Identical Twins.
500
Child HLA Type: A1, A92, B7, B41, C12, C14 Mother HLA Type: A14, A92, B7, B7, C14, C22 Who is the Father? Possible Fathers: 1st HLA Type: A1, A13, B8, B18, C33, C54 2nd HLA Type: A1, A1, B7, B44, C22, C66 3rd HLA Type: A1, A34, B41, B41, C12, C66
3
500
Why do you analyze the D-loop of the mitochondrial DNA?
Because it contains 2 hypervariable regions that are useful for ID testing
500
What is one clinical use for DNA analysis?
ID a specimen Prenatal testing organ transplants
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