Excluding probes, buffers and cations, name the four components required for successful PCR
What are:
Primers
dNTPs
Target (DNA or RNA)
Polymerase?
100
The most common BCR-ABL1 breakpoint (DNA breakpoint and protein designation)
What is the major breakpoint cluster region (M-bcr)?
e13a2 or e14a2
p210
100
FR1, FR2 and FR3 are parts of this
What is each V region in the IGH@ gene?
100
Tissue fixatives which aren't good for molecular studies
What is B5?
Will also take Bouins
Decalcification is an iffy answer, at best (not a "fixative").
100
The only two hematopathology tests that Emory performs on RNA
What is BCR-ABL1 t(9;22) and PML-RARA t(15;17)?
200
Enzyme that helps remove protein from samples during extraction
What is Proteinase K?
200
BCR-ABL1 breakpoint seen more commonly in patients with ALL
What is m-bcr (e19a2; p190)?
200
Sequence in which T-cell genes rearrange
What is delta --> gamma --> beta --> alpha?
200
Tissue fixative generally considered better than formalin for molecular studies
What is Ethanol?
200
Name an assay performed at Emory by pyrosequencing technology
What is BRAF?
What is KRAS?
Either answer is acceptable.
300
Divalent cation which must be present for polymerase chain reaction amplification to occur
What is Magnesium?
300
A leukemia has bone marrow morphology of acute promyelocytic leukemia. However, PCR of PML-RARA fusion gene is negative. Assume that all laboratory test steps are verified correct, this is the most likely explanation
What is variant translocations involving RARA on 17q12?
300
Mobile genetic element that can delete and insert itself variably into the genome
What is a transposon?
300
Charge of DNA at neutral pH
What is negative?
300
The most commonly ordered molecular test at Emory
What is GC/Chlamydia?
400
A260/280 ratio range desired for molecular testing
What is 1.7 - 2.0?
400
The most common type of mutation in the NPM1 gene that occurs in acute myeloid leukemia
What is 4 bp insertion in exon 12 (frameshift)?
400
Sequence of DNA that can affect transcription of a gene far away from it (either 5' or 3')
What is an enhancer?
400
Name all three steps of a PCR cycle
What is denaturation, annealing and extension (polymerization)?
400
Number of board-certified molecular genetic pathologists at Emory
What is four?
Charles Hill, MD, PhD
Karen Mann, MD, PhD
Alexis Carter, MD
Shiyong Li, MD, PhD
500
Method used by MagnaPure to extract nucleic acids
What is magnetic beads?
500
Term for a large B cell lymphoma with both IGH@-BCL2 and MYC translocations
What is "double-hit" lymphoma?
500
Full name of "CDR" in the IGH@ V gene
What is Complementarity Determining Region?
500
Primary method used to rule out the presence of an inhibitor
What is dilute-and-repeat?
500
Assay at Emory which includes a CD15-separation step prior to extraction?