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Groups
AFB
Lab Rats
100

Unpasteurized diary products from infected cows

M. bovis

100

Which characteristic is typical of Mycobacterium species

Waxy cell wall

100

What are the two major groups of Mycobacteria

Mycobacterium tuberculosis complex

 Nontuberculosis mycobacteria

100

What is the most common stain used to identify Mycobacterium species?

Acid-fast stain

100

Name the organism (genus , species) we are looking for when performing a Group B Screen.

Streptococcus agalactiae

200

Can be identified by its susceptibilty to pyrazinamide

M. caprae

200

List 3 sample that could be submitted for an AFB culture

  • Blood-special culture bottle

  • Resp- sputum, wash, lavage, 

  • wound blood urine 

  • fecal

200

Define Complex

2 or more species for which distinction is complicated. Has little to no medical importance

200

M. tuberculosis is a disease of the _____________ tract.

respiratory

200

True or false Group B is a beta hemolytic GNR

false

300

inhalation, transmission from sea lions to humans in zoologic setting

M. pinnipedii

300

When processing an AFB specimen what biosafety cabinet is required?

BSC II

300

The only known noncultivatable nontuberculosis mycobacterium is___.

M.leprae- Hanses disease

300

M. tuberculosis is able to multiply intracellular in the ____________.

Macrophages

300

What is the expected result of a catalase test for GBS?

negative

400

Develop pigment whether grown in light or dark

Scotochromogens

400
Name the stain used to gram stain mycobacterium and other acid fast bacterium.

 Ziehl-Neelsen

400

Name the 4 groups of the nontuberculosis mycobacteria

  • Photochromogens

  • Scotochromogens

  • Nonphotochromogens

  • Rapid growers

400

True or False mycobacteria are usually considered fast growers.

False

400

Which confirmatory test shows arrowhead hemolysis when GBS is streaked near Staphylococcus aureus?

CAMP test

500

Nonpigmented

Nonphotochromogens

500

Name a method used to decontaminate AFB specimens

  • N-acetyl-L-cysteine (NALC) added to NAOH

  • NaOH – decontaminant, emulsifier;NALC  liquifies,

  • NaOH – used alone in higher amounts can liquify and decongest but may inhibit the AFB you are trying to recover

500

List 2 methods used to prelim Identify mycobacterium

Growth Rate, colony morphology, colony texture, pigmentation

500

Name an example of a solid media used to cultivate AFB specimens

  • Agar based(10-12days):Middlebrook

  • Egg-based:(18-24 days): LJ Lowenstein Jensen

500
  • Why is it important to identify GBS in pregnant women?

baby

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