Non-Fermenting Organisms (Unit 4)

General Characteristics

100

What is the Gram Stain Morphology of common Non-Fermenting organisms such as Pseudomonas aeruginosa or Acinteobacter baumannii?

Gram Negative Rods.

200

What is the name of Transport Media used when collecting stool specimens?

Cary-Blair

300

What is the pigment called that makes Pseudomonas aeruginosa blue?

Pyocyanin

400

The aquatic loving organism is called?

Aeromonas

500

Why are Non-fermenters called Non-fermenters? They fail to ferment what substance?

Carbohydrates

Special Requirements

100

What is the atmospheric types required for the growth of Campylobacters?

Microaerophilic & Capnophilic.

200

What is ideal temperature requirements for the growth of Campylobacter jejuni.

42*C.

300

To help identify this organism, use agars with an H2S indicator because this organism is positive.

Shewanella

400

Unique requirements are needed in the cell walls of Shingobacterium spp. What is the component required?

Sphingophospholipids

500

Name the differential media used to identify sucrose fermenting Vibrio from non-fermenting sucose Vibrio organisms.

TCBS.

Biochemical Testing

100

What is the biochemical test that differentiates Enterics from Non-Fermenting Organisms?

Oxidase

200

If a patient is suspected of an H. pylori infection, what test is a definitive clue in the identification process?

Rapid Urease Positive via Clo test

300

What is the sole purpose of performing Oxidative-Fermentation medium on Non-Fermenting GNRs?

To determine if organisms oxidize or ferment carbohydrates, most organisms in this unit are oxidizers meaning they can only utilize the carbohydrates available in the "open" or O2 tube.

400

Another common test performed (but not discussed) is Nitrate Reduction testing. Used in the identification process of Pseudomonas aeruginosa. However, when is Zinc added during this procedure?

After the initial test is negative: Zinc is added to test if the organism broke down Nitrate into Nitrogen gas. Once Zinc is added a color change from clear to pink/red is a negative result.

500

What is the exhibited motility of Campylobacter in a Wet Prep

Darting motility

Causative Agents

100

What is the causative agent of Rice Water Diarrhea that occurs for a consecutive 10 to 30 days? What is the actual name of the disease?

Vibrio cholerae / Cholera

200

What is the causative agent of gastric ulcers?

Helicobacter pylori

300

What is the causative agent of Swimmer's ear, nosocomial pneumonia, & ecthyma gangrenosum.

Pseudomonas aeruginosa

400

What is the causative agent of glanders?

What is the causative agent of melioidosis?

Burkholderia mallei

B. pseudomallei

500

What is the causative agent of tracheotomy infections?

Acinetobacter sp.

Name That Organism

100

This organism grows on routine agars: Beta hemolytic on BA with a greenish-metallic sheen; non-lactose fermenter on MAC; has a grape like smell. Oxidase positive and an OF media Oxidizer.

Pseudomonas aerguinosa.

200

This organism grows on a Campy-BAP at 42*C. The colonies are non-hemolytic, moist, with a runny looking consistency. The gram stain revealed tiny GNRs in S - shapes and seagull wings.

Campylobacter.

300

This organism is called the "salt loving" (halophilic).

Vibrio spp.

400

This organism is found in the tropics, that usually causes infections in the immunocompromised and produces a violet pigment on nonselective media.

Chrombacterium violaceum

500

These organisms are considered potential bioterroristic agents.

Burkholderia mallei & pseudomallei