Run Planning
A maximum of 4 configuration.
How many configurations are available for a local run setup?
The two cloud analysis solutions that are offered with the NovaSeq X.
What are BSSH and ICA?
It has to be replaced every three months.
How often does the air filter have to be replaced?
This is used to determine over- or underloading.
What is %occupied vs %pf?
No, only locally planned runs will be visible.
Can I see cloud planned runs when I log in to the instrument locally?
This is not possible while sequencing is ongoing.
Can any analysis be performed while another sequencing run is ongoing?
Every two weeks.
How often does the maintenance wash have to be performed?
A mandatory requirement for the network connection.
What is the 10GB network switch?
It's automatically generated for every planned run.
What is a samplesheet?
It requires an enterprise account and a domain.
What are the requirements to use ICA?
A known issue with the fluidics.
What is clogging?
Check this secondary metric to optimize loading concentration.
What is the duplication rate?
Up to 8 library prep kits and index adapter kits.
How many different configurations can you chose per DRAGEN App configuration?
A significantly smaller compression format for FASTQs.
What is ORA?
20 uses or 15 months from manufacturing.
What is the life span of a wash flow cell?
This is where the InterOp folder and RunInfo.xml files are located.
What is the usr/local/illumina/mnt/runs folder?
DRAGEN analysis app, library prep kit, index adapter kit.
What are the three necessary settings per analysis configuration?
You will find information about the samples that completed and failed the analysis workflow in these three files.
What are the json files: highlevel-summary.json, detailed-summary.json and workflow-status.json?
They are necessary to clean flow cell and flow cell stage.
What are polynit heatseal wipes?
It allows reagents to be shipped at room temperature.
What are the lyophilized reagents (FFNTPs and ExAMP)?