Run Planning
A maximum of 12+1 configuration.
What is the maximum number of configurations available for a cloud run setup?
The two cloud analysis solutions that are offered with the NovaSeq X.
What are BSSH and ICA?
It has to be replaced every three months.
How often does the air filter have to be replaced?
These primary metrics are used to determine over- or underloading in SAV.
What is %occupied vs %pf?
A .csv file containing information about samples and analysis settings.
What is a samplesheet?
This is not possible while sequencing is ongoing.
What is the requeue of an analysis?
Every two weeks.
How often does the maintenance wash have to be performed?
A mandatory requirement for the network connection.
What is the 10Gb network switch?
Runs you see when logged into the CSW with the cloud account.
What are locally and cloud planned runs?
It requires an enterprise account and a domain.
What are the requirements to use ICA?
Fluidics, Precision motion and RBA movement are checked for troubleshooting.
What are the system checks?
Check this secondary analysis metric to optimize loading concentration.
What is the duplication rate?
Information you need to specify regarding the sample preparation and sample identification
What are the [32] library prep kits and index adapter kits?
A significantly smaller compression format for FASTQs.
What is ORA?
20 uses or 15 months from manufacturing.
What is the life span of a wash flow cell?
This is where the InterOp folder and RunInfo.xml files are located.
What is the usr/local/illumina/mnt/runs folder?
DRAGEN analysis app, library prep kit, index adapter kit.
What are the three necessary settings per analysis configuration?
You will find information about the samples that completed and failed the analysis workflow in these three files.
What are the json files: highlevel-summary.json, detailed-summary.json and workflow-status.json?
They are necessary to clean flow cell and flow cell stage.
What are polynit heatseal wipes?
They are freeze-dried and allow reagents to be shipped at room temperature.
What are the lyophilized reagents (FFNTPs and ExAMP)?