Amino Acids
Which amino acids have more than one chiral center?
Threonine and Isoleucine
What are the optional domains in NRPs
Cy, Ox, Red
Which lab produced the paper
Hilvert
Which amino acid is involved in many Schiff base reactions?
Lysine!
What are the core NRPs domains
A, C, PCP, (TE)
Which domain are they modifying
A domain of TycA and Tyc B (module 3)
What are the specific chiral centers from question 1
Isoleucine is S
Threonine is R
What is thaizoline derived from?
cysteine
What is the specific mutation being put into tyrocidine and why is it important/useful?
Trp-to-Ser
Allows different substrates to bind by increasing binding space
Drawn on board
Which was the first commercially available antibiotic?
Tyrocidine
Why and how did they move to position 4 after trying position 1?
Why - position 1 had side products
How - There was great conservation in amino acids binding to L-Phe at position 4 as well so they made a similar mutation of Trp-to-SerDraw out the major basic amino acids
Drawn on board
What kind of bond / linkage does PCP use?
thioester
What two substituents and metal catalyst were required for the cycloaddition step?
Azide, alkyne, and Cu catalyst