Simplified Order of Draw
Additives
Tests
Departments
Yields Plasma or Serum??
100

I'm the first tube in the order of draw

blood culture bottles (or yellow SPS tube)

100

Purple (lavender) and Pink top tubes contain this additive

EDTA

100

CBCs, HgA1C, ESRs, hemoglobin, and hematocrits (H&H) are all done with this color tube

Lavender

100

Tests utilizing light blue top tubes are used for this department

Coagulation

100

Green top tubes

Plasma

200

I'm the second tube in the order of draw

light blue

200

Yellow top tubes contain this additive (name both)

sodium polyanethol sulfonate (SPS)

acid citrate dextrose (ACD)

200

This tube is used to test for both alcohol and glucose determinations.

Gray top tube

200

Lavender top tubes are related to tests for this department. 

Pink top tubes can be used for this department.

Hematology

Blood banking

200

light blue top tubes

Plasma

300

We're the serum tubes drawn after a light blue top. (name all 2)

serum tubes (red and tiger top/gold)

300

The green top tube contains this additive

sodium or lithium heparin (with or without gel separators)

300

Prothrombin time (PT), activated partial thromboplastin time (aPTT), and D Dimer are drawn on this tube

light blue

300

Serum tubes and gray tubes can all go to this common and comprehensive department

Chemistry

300

Red top, SST (tiger top or gold)

serum

400

We're drawn immediately before the EDTA tubes in the order of the draw

green heparin tubes

400

Light blue top tubes contain this additive

sodium citrate

400

This tube is used for STAT chemistry tests because it can be spun right away. No need to wait for clotting!

Green top heparin tube

400

Yellow SPS tubes go to this department for blood cultures.

Microbiology

400

Lavender and Pink

Plasma 

(whole blood if not spun.  Lavender tubes are the MOST frequently NOT spun tubes) 

500

These tubes are second to last in the order of draw.  (give examples of tubes with this additive)

EDTA tubes (lavender, pink)

500

These are the additives in a gray top tube...

And these are the additives in a red top...

And a SST/tiger top/gold top...


sodium fluoride (glycolytic inhibitor) & potassium oxalate (anticoagulant)

clot activator

clot activator and gel separator



 

500

This group of tubes really do a lot and are used the most often (for routine draws, not STAT). They are used for comprehensive metabolic panels (CMP), Basic Metabolic Panel (BMP) thyroid, prostate specific antigens (PSA), lipids, cardiovascular and hepatic panels.

Serum tubes which include red top tubes and serum separator tubes (SSTS) in tiger top or gold top

500

Tubes drawn on STAT heparin tubes are usually delivered  to this department?

Chemistry

500

Gray top

plasma with potassium oxalate (whole blood if not spun)

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