PCR STEPS 1
The first step of PCR
What is Denature
In order what are the 3 steps of PCR
Denature, Anneal, Extension
RT-PCR stands for
Reverse Transcription- Polymerase Chain Reaction
PCR is short for
Polymerase Chain Reaction
An enzyme is this biomolecule
What is a protein
The temp required for this step is 72 degrees Celsius
What is Extension
The third step of PCR
What is Extension
This type of PCR is also known as real-time PCR
What is qPCR or quantitative PCR
A piece of DNA that the nucleotides get added to
What is a Primer
The 3 components of a DNA nucleotide
What is nitrogenous base, deoxyribose sugar, and a phosphate group
The second step of PCR
What is annealing
2 primers are required for this step
Annealing
This type of PCR allows for the simultaneous amplification of multiple DNA fragments in a single reaction
What is Multiplex PCR
The region of DNA that we want to duplicate for analysis
Target sequence or template
Transcription takes place in this cell organelle
What is the nucleus?
This enzyme is required for Extension
What is DNA polymerase/Taq Polymerase
During which step a sample is heated to separate DNA into two single strands.
What is Denature
A type of PCR that measures the exact number of nucleic acid copies in a sample
What is dPCR or digital PCR
Another term for nucleotides
What is bricks
Translation takes place in this cell organelle
What is ribosome
The temperature required for annealing is
between 45-60 degrees Celsius
True or False: DNA Polymerase can copy DNA from the 5' to 3' end
What is false
Most sensitive technique for mRNA detection and quantitation currently available.
rt-PCR
A single segment or small sample of DNA is amplified to generate thousands of copies for analysis during this process
What is PCR
This molecule can leave the nucleus
Wat is mRNA