Equipment
Needle Components
Blood Collection Supplies
Venipuncture Technique
Safety & Infection Control
100

This elastic band is placed above the venipuncture site to make veins more visible.

Tourniquet

100

This part of the needle is inserted directly into the vein.

Bevel

100

This tube color contains no additive and is commonly used for discard purposes.

Red-top tube

100

This is the recommended angle of needle insertion for routine venipuncture.

15–30 degrees

100

This action must be performed before and after every patient contact.

Hand hygiene (handwashing)

200

This device holds the needle and collection tube together during venipuncture.

Needle holder (tube holder)

200

This needle component controls blood flow and prevents leakage before tube insertion.

Rubber sleeve (Sheath Covering)

200

This tube color contains EDTA and is commonly used for hematology tests.

Lavender (purple) top

200

This vein is the preferred choice for routine blood draws.

Median cubital vein

200

This container is used for immediate disposal of used needles.

Sharps container

300

This piece of equipment is used to collect blood when veins are fragile or small.

Butterfly needle (winged infusion set)

300

This part of a multisample needle allows multiple tubes to be filled with one puncture.

Double-ended needle

300

This tube additive prevents blood from clotting by binding calcium.

EDTA

300

This step must be done immediately after withdrawing the needle to prevent bruising.

Apply pressure to the site

300

These precautions are used for all patients regardless of diagnosis.

Standard precautions

400

This collection method is preferred when multiple tubes must be drawn while minimizing vein collapse.

Vacuum tube collection system

400

Using a needle gauge that is too small increases the risk of this blood specimen error.

Hemolysis

400

This tube additive allows blood to clot and is commonly used for serum tests.

Clot activator

400

Prolonged tourniquet application can cause falsely elevated levels of this due to hemoconcentration.

Potassium (also calcium, protein, hematocrit acceptable)

400

This action must never be performed after using a needle.

Recapping a needle

500

This blood collection system uses negative pressure to draw blood into tubes automatically.

Vacuum tube collection system (Vacutainer system)

500

A needle larger than 18 gauge or smaller than 23 gauge increases the risk of specimen rejection due to this issue.

Hemolysis or vein trauma

500

Place these tubes in the correct order of draw: blood cultures, lavender, light blue, serum, gray.

Yellow → Light blue → Red → Gold/Tiger → Green → Lavender → Gray

500

This error can cause hemolysis due to excessive suction or improper technique.

Using too much pressure or pulling back too forcefully on the syringe (Petechia)

500

This federal agency regulates bloodborne pathogen standards in healthcare settings.

OSHA (Occupational Safety and Health Administration)

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