Plasmid that is engineered to make a more useful tool for molecular biologists.
What is a Vector
100
name of place where DNA has been cleave.
What is Sticky ends.
100
two most common vectors used
What is bacteriophage λ and E.Coli
100
all the DNA insertions are the same
What is False
200
replicate (a fragment of DNA placed in an organism) so that there is enough to analyze or use in protein production.
What is a clone
200
Piece that is inserted into Plasmid
What is DNA fragment.
200
The Three sites on a plasmid
What is replication origin, ampicillin-resistance gene, DNA insertion region
200
All E.Coli bacteria live after combination with anti-biotic
What is False
300
a genetic structure in a cell that can replicate independently of the chromosomes, typically a small circular DNA strand in the cytoplasm of a bacterium or protozoan
What is a plasmid
300
Type of bacteria added to plasmids in the animation
What is E.Coli
300
Part of the cell that fights off anti-biotic
What is ampicillin-resistance gene
300
Not all plasmids become inserted within the E.Coli
What is True
400
a semisynthetic form of penicillin used chiefly to treat infections and forms of E.coli
What is ampicillin
400
enzyme that seals the insertion of the DNA fragment
What is DNA Ligase
400
shape of a plasmid
What is Circular
400
Plasmids replicate separately from the rest of the cell
What is true.
500
a particular sequence in a genome at which replication is initiated. This can either involve the replication of DNA in living organisms such as prokaryotes and eukaryotes, or that of DNA or RNA in viruses.
What is a replication origin
500
the sequence of nucleotides on the sticky ends
What is GAATTC
500
Plasmids are only located in this type of cell
What is Prokaryotic
500
Transformation is the introduction of recombinant DNA into recipient sells