Stains
Slants
Plates
Deeps and others
Microscopes and aseptic technique
100
Which color is the Gram-positive?
Purple
100
For the phenylalanine test, slant ___ contains an organism that can produce the enzyme deaminase
Top slant
100
Streak ___ is positive for the production of the enzyme DNase.
Right
100
What is the indicator of a catalase positive test?
Bubble formation
100
How should you transport the microscope?
Hold the arm and the base
200
What does the negative stain do and why?
Stains the background because the bacterium is – and the stain is – so it is repelled
200
Tube ___ is negative meaning there was no hydrolysis of urea.
C (or b)
200
What type of organisms will have good growth and why? (MSA)
Gram +, can grow in high salt
200
Test ___ is positive for the oxidase test.
B, right
200
What objective lens should be used for oil immersion?
100x
300
Which organism is Gram negative and what is the shape seen under the microscope?
Pink rod (bacillus)
300
What method do you use to inoculate a TSI slant?
Stab and surface streak
300
Streak ___ exhibits Beta hemolytic growth of an organism that has completely broken down all parts of the red blood cells that were near it in the CNA agar
A
300
Are these tests selective, differential, or both?
differential
300
Why is aseptic technique important?
Safety
400
The Gram ____ bacteria was clear after the decolorization step of the Gram staining process.
negative
400
The tube with bright blue agar can / cannot (circle one) use citrate as its sole source of carbon.
can!
400
Streak ___ has a Gram ________ bacterium growing on it that ferments lactose. 
B or C, negative
400
What are two sugars that could be tested with phenol red fermentation?
Glucose, lactose, or sucrose
400
Where should you dispose of your gloves
Biohazard Safety Bag
500
What is the role of the mordant?
Enhances crystal violet stain
500
What are two types of media that you streak with a superficial inoculation?
Slants and plates
500
Plate ___ contains an organism that is resistant to the antibiotic on the disk.
A
500
Which tube is positive for the SIM indole test?
B
500
In the lab you spill a broth with bacteria growing on it, briefly describe how to correctly clean up the mess. This description should include proper waste disposal instructions.
Cover tube and spill with a paper towel, wet the area with sporklenz, then call over your TA