Step Name
Step Enzyme
Step Product
Step Rationale
Step Mechanism
100

The name of the first step of Glycolysis

What is Phosphorylation of Glucose?

100

The enzyme used in the first step of Glycolysis

What is Hexokinase?

100

The product of the first step of Glycolysis

What is Glucose 6-phosphate?

100

The rationale for the first step of Glycolysis

What is to trap glucose inside the cell and lower intracellular glucose concentration to allow for further uptake?

100

The mechanism for the first step of Glycolysis

What is phosphorylation of glucose by hexokinase/glucokinase to form glucose 6-phosphate using ATP?

200

The name of the second step of Glycolysis

What is Phosphohexose Isomerization?

200

The enzyme used in the second step of Glycolysis

What is Phosphohexose Isomerase?

200

The product of the second step of Glycolysis

What is Fructose 6-phosphate?

200

The rationale for the second step of Glycolysis

What is to make C1 of fructose easier to phosphorylate by PFK and to allow for symmetrical cleavage by adolase?

200

The mechanism for the second step of Glycolysis

What is isomerization of glucose 6-phosphate to fructose 6-phosphate by phosphohexose isomerase?

300

The name of the third step of Glycolysis

What is 2nd Priming Phosphorylation?

300

The enzyme used in the third step of Glycolysis

What is Phosphofructokinase-1 (PFK-1)?

300

The product of the third step of Glycolysis

What is Fructose 1,6-bisphosphate?

300

The rationale for the third step of Glycolysis

What is to further activate glucose and allow for a 1 phosphate, 3 carbon sugar after step 4? 

300

The mechanism for the third step of Glycolysis

What is 2nd phosphorylation by PFK-1 to form fructose 1,6-bisphosphate?

400

The name of the fourth step of Glycolysis

What is Adol Cleavage of Fructose 1,6-Bisphosphate?

400

The enzyme used in the fourth step of Glycolysis

What is Aldolase?

400

The products of the fourth step of Glycolysis

What are Dihydroxyacetone phosphate (DHAP) and Glyceraldehyde 3-phosphate (GAP)?

400

The rationale for the fourth step of Glycolysis

What is to cleave the six-carbon sugar into 2 three-carbon, high-energy phosphate sugars?

400

The mechanism for the fourth step of Glycolysis

What is cleavage of fructose 1,6-bisphosphate by adolase into two 3-carbon sugars, DHAP and GAP?

500

The name of the fifth step of Glycolysis

What is Triose Phosphate Interconversion?

500

The enzyme used in the fifth step of Glycolysis

What is Triose Phosphate Isomerase

500

The product of the fifth step of Glycolysis

What is Glyceraldehyde 3-phosphate (GAP)?

500

The rationale for the fifth step of Glycolysis

What is to allow glycolysis to proceed to the payoff phase by a single chemical pathway, and to ensure that both 3-carbon sugars are now GAPs?

500

The mechanism for the fifth step of Glycolysis

What is isomerization of DHAP to GAP by triose phosphate isomerase, completing the prep phase?

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