Gene Cloning
PCR
Transgenics
Genomics
Proteomics & Bioinformatics
100
method of isolating and making many copies of a gene
gene cloning
100
PCR consists of cycles of denaturation, annealing, and this step.
extension
100
This plasmid is required for plant transformation
Ti plasmid
100
Comparing the genomes of different species
What is comparative genomics
100
This is larger than the genome due to events like alternative splicing.
What is the proteome
200
These enzymes function to make sticky ends
restriction enzymes
200
Thermus aquaticus is the bacteria from which this DNA polymerase is isolated.
Taq polymerase
200
This bacterium is required for transformation in plants.
Agrobacterium tumefaciens
200
By studying the genomics of this animal, we have learned much about human medicine & disease.
What is rhesus monkey
200
This biology field uses computers to analyze genetic data.
What is bioinformatics
300
This enzyme is needed to bond the vector and gene together before bacterial insertion.
DNA ligase
300
RT-PCR utilizes reverse transcriptase to generate these from mRNA molecules
cDNA
300
A gene conferring this is transferred to the plasmid in addition to the gene of interest.
antibiotic resistance
300
sequencing the genome of an entire community
What is metagenomics
300
This common proteomic method can be used to distinguish hundreds of proteins from a cell extract.
What is two-dimensional gel electrophoresis
400
Hybrid vectors are distinguished from recircularized vector by being this color on media.
white
400
This is the reason that denaturation occurs at high temperature
melt the hydrogen bonds between the strands
400
These genes are shut down in the plasmid.
Tumor causing genes
400
determining the role of genetic sequences within a species
What is functional genomics
400
This type of protein microarray uses native cellular proteins to analyze protein binding properties.
What is a functional microarray
500
This dye is needed to create the blue color of recircularized vectors from beta-galactosidase.
X-Gal
500
Extension removes this molecule from the quencher allowing for real-time quantitation of PCR product.
reporter
500
When agrobacterium is unable to work, this method utilizes an electric current to generate pores in the plasma membrane.
electroporation
500
This type of analysis can be used to measure gene expression in different tissues or stages of development
What is a DNA microarray
500
This proteomic technique can be used to analyze an isolated peptide to determine the gene (codon) sequence for it.
What is mass spectrometry
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