Misc.
You subject two different size linear DNA fragments to standard gel electrophoresis with positive cathode at the bottom of gel. What can you predict about their behavior.
Both fragments will move upwards in the gel; the smaller fragment will move further up.
Both fragments will move downwards in the gel; the smaller fragment will move further down.
Both fragments will move upwards in the gel; the larger fragment will move further up.
Both fragments will move downwards in the gel; the larger fragment will move further down.
None of the above, since we don't know the nucleotide sequence and therefore the overall charge of each fragment.
You subject two different size linear DNA fragments to standard gel electrophoresis with positive cathode at the bottom of gel. What can you predict about their behavior.
Both fragments will move upwards in the gel; the smaller fragment will move further up.
Both fragments will move downwards in the gel; the smaller fragment will move further down.
Both fragments will move upwards in the gel; the larger fragment will move further up.
Both fragments will move downwards in the gel; the larger fragment will move further down.
None of the above, since we don't know the nucleotide sequence and therefore the overall charge of each fragment.
Which of the following statements is accurate about transcription of a gene that is expressed at high levels?
The gene has a promoter region that is in complex with the enhancer region of same gene.
The gene does not have a TATA box in its promoter.
The gene has a TATA box that has been moved to its enhancer.
The gene is transcribed once only
None of the above
Which of the following statements is accurate about transcription of a gene that is expressed at high levels?
The gene has a promoter region that is in complex with the enhancer region of same gene.
The gene does not have a TATA box in its promoter.
The gene has a TATA box that has been moved to its enhancer.
The gene is transcribed once only
None of the above
Expression Vector:
A collection of entire genome of population of cells cleaved into fragments, inserted into selectable plasmids and introduced into bacteria
A plasmid with an origin of replication, one or more selectable markers, and a strong promoter
RNA that codes for protein
Sequences/regions of DNA far from a gene whose transcription they regulate and distinct from a promoter region
Expression Vector:
A collection of entire genome of population of cells cleaved into fragments, inserted into selectable plasmids and introduced into bacteria
A plasmid with an origin of replication, one or more selectable markers, and a strong promoter
RNA that codes for protein
Sequences/regions of DNA far from a gene whose transcription they regulate and distinct from a promoter region
Proteins can be denatured and nucleic acids can not be denatured
True
False
Proteins can be denatured and nucleic acids can not be denatured
True
False
Restriction endonucleases
Are Hydrolases that recognize specific short DNA sequences.
Are Enzymes that hydrolyze phosphodiester bonds on RNA molecules during splicing.
Do not denature in high temperatures and are used in PCR reactions for DNA amplification.
Are ligases that recognize specific short sequences.
None of the above
Restriction endonucleases
Are Hydrolases that recognize specific short DNA sequences.
Are Enzymes that hydrolyze phosphodiester bonds on RNA molecules during splicing.
Do not denature in high temperatures and are used in PCR reactions for DNA amplification.
Are ligases that recognize specific short sequences.
None of the above
We have defined cloning a gene as
Making many identical copies of a DNA molecule by PCR.
Making many identical copies of a DNA molecule by inserting it into a plasmid.
Overexpressing protein from an expression vector.
Creating a DNA library of the entire genome using plasmids.
None of the above
We have defined cloning a gene as
Making many identical copies of a DNA molecule by PCR.
Making many identical copies of a DNA molecule by inserting it into a plasmid.
Overexpressing protein from an expression vector.
Creating a DNA library of the entire genome using plasmids.
None of the above
mRNA
A collection of entire genome of population of cells cleaved into fragments, inserted into selectable plasmids and introduced into bacteria
A plasmid with an origin of replication, one or more selectable markers, and a strong promoter
RNA that codes for protein
Sequences/regions of DNA far from a gene whose transcription they regulate and distinct from a promoter region
mRNA
A collection of entire genome of population of cells cleaved into fragments, inserted into selectable plasmids and introduced into bacteria
A plasmid with an origin of replication, one or more selectable markers, and a strong promoter
RNA that codes for protein
Sequences/regions of DNA far from a gene whose transcription they regulate and distinct from a promoter region
On any chromosome, either of the DNA double helix strands may serve as "template" for the transcription of different genes.
True
False
On any chromosome, either of the DNA double helix strands may serve as "template" for the transcription of different genes.
True
False
You isolate total nuclear chromosomes from human cells, remove all the chromatin proteins and perform in vitro metabolic labelling by adding radioactive deoxynucleotide monomers to tag/label a newly synthesized strand of B-globin gene. What are the minimum remaining factors you need to perform that experiment in the test tube?
A primer complementary to a portion of B-globin gene, DNA polymerase, ligase.
Ability to denature and anneal the DNA double strand, DNA polymerase, ligase.
Ability to denature and anneal the DNA double strand, a primer complementary to a portion of B-globin gene, DNA polymerase.
Ability to denature and anneal the DNA double strand, reverse transcriptase, DNA polymerase.
None of the above
You isolate total nuclear chromosomes from human cells, remove all the chromatin proteins and perform in vitro metabolic labelling by adding radioactive deoxynucleotide monomers to tag/label a newly synthesized strand of B-globin gene. What are the minimum remaining factors you need to perform that experiment in the test tube?
A primer complementary to a portion of B-globin gene, DNA polymerase, ligase.
Ability to denature and anneal the DNA double strand, DNA polymerase, ligase.
Ability to denature and anneal the DNA double strand, a primer complementary to a portion of B-globin gene, DNA polymerase.
Ability to denature and anneal the DNA double strand, reverse transcriptase, DNA polymerase.
None of the above
To begin transcription of a gene
RNA polymerase binds at the enhancer region after general transcription factors bind to the TATA box.
General transcription factors & RNA polymerase bind at promoter region while other regulatory proteins bind enhancer region and the two regions physically come together.
Enhancer & promoter regions are first transcribed and then gene is transcribed.
TATA box is spliced out
None of the above
To begin transcription of a gene
RNA polymerase binds at the enhancer region after general transcription factors bind to the TATA box.
General transcription factors & RNA polymerase bind at promoter region while other regulatory proteins bind enhancer region and the two regions physically come together.
Enhancer & promoter regions are first transcribed and then gene is transcribed.
TATA box is spliced out
None of the above
Distant regulatory sequences/regions
A collection of entire genome of population of cells cleaved into fragments, inserted into selectable plasmids and introduced into bacteria
A plasmid with an origin of replication, one or more selectable markers, and a strong promoter
RNA that codes for protein
Sequences/regions of DNA far from a gene whose transcription they regulate and distinct from a promoter region
Distant regulatory sequences/regions
A collection of entire genome of population of cells cleaved into fragments, inserted into selectable plasmids and introduced into bacteria
A plasmid with an origin of replication, one or more selectable markers, and a strong promoter
RNA that codes for protein
Sequences/regions of DNA far from a gene whose transcription they regulate and distinct from a promoter region
The gene for a given eukaryotic protein is transcribed only once into a single complementary mRNA molecule which is then translated repeatedly on polyribosomes in the cytoplasm.
True
False
The gene for a given eukaryotic protein is transcribed only once into a single complementary mRNA molecule which is then translated repeatedly on polyribosomes in the cytoplasm.
True
False
Genomic DNA Library
A collection of entire genome of population of cells cleaved into fragments, inserted into selectable plasmids and introduced into bacteria
A plasmid with an origin of replication, one or more selectable markers, and a strong promoter
RNA that codes for protein
Sequences/regions of DNA far from a gene whose transcription they regulate and distinct from a promoter region
Genomic DNA Library
A collection of entire genome of population of cells cleaved into fragments, inserted into selectable plasmids and introduced into bacteria
A plasmid with an origin of replication, one or more selectable markers, and a strong promoter
RNA that codes for protein
Sequences/regions of DNA far from a gene whose transcription they regulate and distinct from a promoter region
The key enzyme in making a cDNA library is reverse transcriptase, since RNA is the starting material and a complementary DNA needs to be made.
True
False
The key enzyme in making a cDNA library is reverse transcriptase, since RNA is the starting material and a complementary DNA needs to be made.
True
False
If you are studying TATA boxes, you would have to use a cDNA library to find the cloned region.
True
False
If you are studying TATA boxes, you would have to use a cDNA library to find the cloned region.
True
False