Background
Calling Genes I
Calling Genes II
Central Dogma
Phamerator
100
A non-living microscopic parasite
What is a phage? *NGRI Phage Resource Guide. Chevy Chase: HHMI, 2010.
100
This, abbreviated ORF, is the region between a start and stop codon that potentially codes for a protein.
What is an open reading frame? *NGRI Phage Resource Guide. Chevy Chase: HHMI, 2010.
100
A gene should be more than this many codons long
What is 50? *NGRI Phage Resource Guide. Chevy Chase: HHMI, 2010.
100
The central dogma of molecular biology consists of these two processes
What are transcription and translation? *NGRI Phage Resource Guide. Chevy Chase: HHMI, 2010.
100
This is the name for a family of phages
What is a phamily? *NGRI Phage Resource Guide. Chevy Chase: HHMI, 2010.
200
Phages infect these
What are bacteria? *NGRI Phage Resource Guide. Chevy Chase: HHMI, 2010.
200
ATG and GTG are the two most common examples of these Bonus: This is the third most common start codon
What are start codons? Bonus: What is TTG? *NGRI Phage Resource Guide. Chevy Chase: HHMI, 2010.
200
BLASTx results should have high these
What are max scores? *NGRI Phage Resource Guide. Chevy Chase: HHMI, 2010.
200
During transcription, a strand of this is built from DNA
What is mRNA? *NGRI Phage Resource Guide. Chevy Chase: HHMI, 2010.
200
Related phages are organized into groups called these, e.g. A5
What are clusters? *NGRI Phage Resource Guide. Chevy Chase: HHMI, 2010.
300
A phage is a type of this
What is a virus? *NGRI Phage Resource Guide. Chevy Chase: HHMI, 2010.
300
AGGAGGA is the consensus sequence of this region, which should be 4-18 base pairs upstream of the start codon
What is the Ribosome Binding Site, or Shine-Dalgarno sequence? *NGRI Phage Resource Guide. Chevy Chase: HHMI, 2010.
300
BLASTx results should have low these
What are E values? *NGRI Phage Resource Guide. Chevy Chase: HHMI, 2010.
300
This enzyme is responsible for binding to a promoter site and then "unzipping" strands of DNA
What is RNA polymerase? *James Sandoz and Steve Caruso. “Gene Structure.” 7 February 2011. BIOL 216H.
300
This tool graphically displays to what degree the phages in a phamily are related
What is a pham circle? *NGRI Phage Resource Guide. Chevy Chase: HHMI, 2010.
400
Phages store their genetic information in this part of their anatomy
What is a capsid? *NGRI Phage Resource Guide. Chevy Chase: HHMI, 2010.
400
The amount of base pairs that two genes overlap should be less than this
What is 20? *NGRI Phage Resource Guide. Chevy Chase: HHMI, 2010.
400
A good BLASTx result should include at least one of these, for example phosphotransferase
What is a functional protein? *NGRI Phage Resource Guide. Chevy Chase: HHMI, 2010.
400
This group of three base pairs codes for an amino acid
What is a codon? *James Sandoz and Steve Caruso. “Gene Structure.” 7 February 2011. BIOL 216H.
400
According to Phamerator, Methuselah is most related to Bxz2, which belongs in this cluster
What is A3?
500
This benign bacterium that we used to isolate Methuselah is related to Tuberculosis
What is Mycobacterium smegmatis?
500
This bioinformatics tool, named after the heir of Isildur in the Lord of the Rings trilogy, is used to find tRNA.
What is ARAGORN? *NGRI Phage Resource Guide. Chevy Chase: HHMI, 2010.
500
This type of RNA removes a ribosome from the RNA strand when there is no stop cocon
What is tmRNA? *NGRI Phage Resource Guide. Chevy Chase: HHMI, 2010.
500
Amino acids connect into these, which form proteins
What are polypeptide chains? *James Sandoz and Steve Caruso. “Gene Structure.” 7 February 2011. BIOL 216H.
500
When you compare two genomes in the Map feature of Phamerator, this color represents a 100% correlation between the two
What is purple? *NGRI Phage Resource Guide. Chevy Chase: HHMI, 2010.
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