EASY
What is the purpose of microscopy in biological methods?
a) To separate DNA fragments
b) To observe cells and tissues
c) To amplify DNA
d) To measure protein concentration
b) To observe cells and tissues
Describe the difference between agarose electrophoresis and polymerase chain reaction (PCR).
a) Agarose electrophoresis amplifies DNA, while PCR separates DNA fragments.
b) Agarose electrophoresis separates DNA fragments, while PCR amplifies DNA.
c) Both agarose electrophoresis and PCR are used to detect proteins.
d) Agarose electrophoresis studies crystal structures, while PCR amplifies RNA.
b) Agarose electrophoresis separates DNA fragments, while PCR amplifies DNA.
Explain the importance of aseptic techniques in biological methods and provide an example of its application.
a) It prevents contamination of biological samples; used in tissue culture.
b) It separates protein mixtures; used in enzyme assays.
c) It ensures accurate pH measurements; used in spectrophotometry.
d) It enhances DNA visibility; used in agarose electrophoresis.
a) It prevents contamination of biological samples; used in tissue culture.
Name one example of a chemical method used to determine the concentration of a solution.
a) Distillation
b) Titration
c) Centrifugation
d) Drying
b) Titration
What are the main principles behind titration and chromatography in chemical methods?
a) Titration measures heat flow, and chromatography identifies DNA.
b) Titration separates mixtures, and chromatography measures temperature changes.
c) Titration measures concentration, and chromatography separates mixtures.
d) Both titration and chromatography identify protein antigens.
c) Titration measures concentration, and chromatography separates mixtures.
How does X-ray diffraction (XRD) help determine the crystal structure of substances in physical methods?
a) By measuring electron interactions in magnetic fields.
b) By determining the arrangement of atoms based on scattered X-rays.
c) By analyzing light absorbance patterns of a substance.
d) By separating different types of crystals using density gradients.
b) By determining the arrangement of atoms based on scattered X-rays
What is centrifugation used for in physical methods?
a) To measure pH
b) To separate substances by density
c) To identify chemical compounds
d) To detect proteins
b) To separate substances by density
How does spectrophotometry help in analyzing chemical substances?
a) By amplifying specific DNA segments.
b) By detecting antigens using an enzymatic reaction.
c) By observing physical changes under a microscope.
d) By measuring how substances absorb or emit light.
d) By measuring how substances absorb or emit light.
Compare the uses of thermal analysis and rheometry in analyzing physical properties of materials.
a) Thermal analysis measures heat flow, while rheometry studies deformation and flow.
b) Thermal analysis separates mixtures, while rheometry amplifies light scattering.
c) Both measure electrical conductivity in substances.
d) Both are used to analyze density changes in liquids.
a) Thermal analysis measures heat flow, while theometry studies deformation and flow.
What is the main purpose of agarose electrophoresis?
a) To measure chemical concentration
b) To separate DNA fragments by size
c) To observe cells under a microscope
d) To grow cells outside their natural environment
b) To separate DNA fragments by size
Which physical method can be used to determine the pH of a solution?
a) Centrifugation
b) Chromatography
c) Weighing
d) pH measurement
d) pH measurement
Why is tissue culture important in biological research?
a) It separates chemical mixtures by physical properties.
b) It allows growth and study of cells in a controlled environment.
c) It measures the heat flow of cellular activities.
d) It determines the density of cellular components.
b) It allows growth and study of cells in a controlled environment.