Cryosection
The brains we use during sectioning are derived from these two animals.
What are rats and mice
Prior to staining, slide are stored at this temperature.
What is -80°C
What is genotyping
OR to analyze DNA
OR to isolate segments of interest
What is 2,987
This plane view is sectioned vertically, separating the anterior half from the posterior half of the brain.
What is a coronal section
This is what the acronym, OCT, stands for.
What is Optimal Cutting Temperature
An antigen is a toxin or foreign substance that induces an immune response, resulting in the production of antibodies. This site, located on the antigen, is where the antibody specifically binds.
What is an epitope
The further down the DNA travels through the gel, the ____ it is.
What is smaller
This is the purpose of the secondary antibody.
What is detecting the primary (bonus: the secondary antibody signals to the researcher if the target protein is present)
This plane view is sectioned vertically, separating the right and left brain.
What is a sagittal section
The cryostat should be set at this temperature.
What is -20°C
This is the name of a technique used to harvest antibodies by injecting an animal with an antigen, thereby provoking an immune response.
What is Hybridoma
This is the name of the machine that facilitates the three basic steps of PCR: denaturation, extension, and annealing.
What is a thermal cycler
This is a reason for using a colored dye when loading a SDS-PAGE gel.
What is "the dye helps the protein sink to the bottom of the well"
OR the dye allows the researcher to see the protein leaving their pipette and entering the well correctly
This lobe contains the hippocampus, which is involved in learning and memory.
What is the temporal lobe
Tissue sections are sliced in terms of this unit of measurement.
What are micrometers
This is the name of a blocking agent, used to reduce false-positive staining of amino acids other than those within the desired epitope.
What is animal serum
OR goat serum
OR blocking buffer
OR diluted DAPI
DNA is ___ charged, and therefore runs towards the ___ end of the gel chamber.
This is the order of all the materials that go into the transfer apparatus, starting from the negatively charged side.
What is "black side, sponge, filter paper, gel from SDS, synthetic membrane, filter paper, sponge, clear side"
This part of the brain is involved in postural adjustment, maintaining balance, and muscle coordination.
What is the cerebellum
Tissue sections stick to the slides because of this.
What are opposite charges
This is the name of the device, used in confocal fluorescence microscopy, that converts small amounts of light energy into large amounts of electrical energy.
What is a photomultiplier tube
This organism is where our polymerase comes from (full name and genus)
What is thermus aquaticus, bacterium
What is 0.45
These structures make up the forebrain.
What is the telencephalon and diencephalon