RFLP
RFLP Analysis
RFLP Linkage Maps
RFLP in Agriculture
100
What does RFLP stand for?
What is Restriction Fragment Length Polymorphism.
100
How many steps are there in RFLP analysis?
There are 6 steps.
100
Do RFLP's play a role in creating genetic linkages? Yes or No
Yes
100
What number of major crops species use RFLP for development?
14 major crop species.
200
What is RFLP?
RFLP is any difference in homologous DNA sequences.
200
What is RFLP analysis?
RFLP analysis involves the comparison of different lengths of DNA fragments to determine the genetic difference between individuals.
200
What happens in RFLP maps?
In RFLP maps, special enzymes are used to cut the DNA of an organism into smaller pieces.
200
Since ________, restriction fragment length polymorphism has emerged as a very powerful tool for genetic analysis in plants.
1985
300
Where can RFLP's be used?
In paternity cases or criminal cases to determine the source of DNA as a sample.
300
What are the names of two restriction enzymes?
EcoRI and HaeIII.
300
Does RFLP mapping reveal some kind of pattern? Yes or No
Yes
300
What makes RFLP a very important tool in plant genetics?
The ability to detect variation directly at the DNA level.
400
What can RFLP's be used to determine?
The disease status of an individual.
400
What is a restriction enzyme?
A restriction enzyme is an enzyme that cuts double-stranded or single-stranded DNA at sites known as restriction sites.
400
What kind of information do the patterns reveal, by probing the cut DNA?
They can be used to determine linkage of traits to specific RFLP patterns.
400
Where have RFLP techniques been exploited?
Indian rice and rape mustard programs.
500
Where do polymorphisms occur?
In coding and non-coding regions.
500
What is a southern blot?
Southern blotting is a method used to visualize specific segments of DNA, usually a particular gene.
500
What does establishing both of these linkages allow?
Establishing these linkages allows both animal and plant researchers to more efficiently develop organisms of increased agricultural performance.
500
What are the crop species under study using RFLP's?
Monocots and Dicots.
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