Row 1
What is the principle of the starch test? What organism was positive?
Enzyme - Amylase
Breaks down the starch around creating a clear/lighter zone
Iodine binds to starch to form a dark coloured complex
What is the function of glycerol when freezing? Why?
Used for long term storage. Prevents formation of icicles
Read this SIM result?
Answer varies
Calculate the VCN in image on the board
On vibes
What is the indicator in glucose/lactose fermentation?
What is the indicator in urea?
Phenol red
Methyl red.
Bonus: What do the colour changes mean?
What are the steps in PCR?
What is the principle of the oxidase test? What organism used in lab was positive? Negative?
-
Machine used for PCR is?
Thermal cycler
What is a heterocyst?
Found in cyanobacteria. Used for nitrogen fixation
What was the point of the epidemiology lab?
Reminder for A: Epidemiology results
How do you cross out errors during record keeping?
Single horizontal line
Phycology is the study of ?
Algae
What is BLAST?
-
Principle of gel electrophoresis?
Charge of DNA
Charge of electrodes
Migration
Functions of gel green dye and loading dye
Gel green dye - allows visualization of DNA bands
Loading dye has two components; bromophenol blue and glycerol
What is conjugation? What did we do in the conjugation experiment? Focus on the bacteria we used, the plasmids, the auxotroph, prototroph etc
Direct transfer of genes via pilus. PAW-15 & 503CA.
Reminder for A: Go over results
Lysogenic vs lytic cycle. How do the plaques usually look?
Cloudy vs clear
What is transformation? What did we do in the transformation experiment?
E.g What was lysed
Uptake of bacteria from the environment.
Protocatechuate oxygenase
What are the buffer components in the transformation experiment and what do they do?
TENS: Tris-HCl, EDTA, NaOH, SDS
What is this organism
Varies