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In eukaryotes, this enzyme synthesizes messenger RNA from a DNA template.
What is RNA polymerase II?
In contrast to gene knockout, this genetic technique involves inserting a functional gene at a specific locus.
What is gene knock-in?
In RNAi, this enzyme processes long double-stranded RNA into short siRNA molecules.
What is Dicer?
This small mammal is frequently used in gene knockout studies to model human diseases.
What is the mouse
This mosquito species is the primary vector for viruses like dengue, Zika, and chikungunya.
What is Aedes aegypti?
This molecule brings amino acids to the ribosome during translation.
What is transfer RNA (tRNA)?
My effect is permanent and heritable
what is gene knock-in
The RNA-induced silencing complex (RISC) contains this protein, which cleaves mRNA to prevent translation.
What is Argonaute?
Gene knockouts created with CRISPR often rely on this error-prone DNA repair pathway.
What is non-homologous end joining (NHEJ)?
This mechanism is used to suppress gene expression in insects
What is RNA interference (RNAi)?
The three-letter sequences in mRNA that code for amino acids are called this.
What are codons?
I guide the cas 9 enzyme to make a cut on the DNA
what is a gRNA?
This synthetic RNA molecule is commonly used in gene knockdown experiments to silence genes.
What is small interfering RNA or siRNA?
Knocking out the p53 gene in mice is a common model for studying this disease.
What is cancer?
This factor was found to improve knockdown efficiency by reducing variability between replicates in Aedes aegypti.
What is the use of transfection reagents (such as Cellfectin II)?
This Shakespearean play features the famous quote, “To be or not to be.”
What is Hamlet?
I provide the sequence needed to be inserted in the target DNA during gene Knock-in
What is a donor sequence?
A major disadvantage of RNAi-based gene knockdown is the potential problem, where unintended genes are silenced.
What is off-target effects?
This system uses Cre recombinase and loxP sites to create tissue-specific gene knockouts.
What is the Cre-Lox system?
This molecule was ineffective at silencing genes in adult Aedes aegypti mosquitoes.
What is small interfering RNA (siRNA)?
The chemical symbol for gold is this.
What is Au?
A type of gene intervention that causes a gain in function.
What is gene knock-in
This is the main difference between siRNA and miRNA
what is target-specific binding
CRISPR-based gene knockouts often rely on this DNA repair pathway to introduce precise insertions via donor templates.
What is homology-directed repair (HDR)?
Due to the increasing resistance of Aedes aegypti to this type of control method, alternative gene-targeting approaches are being explored.
What are insecticides?