Sentinel animal details
Process
Collection
Numbers
Misc
100
These are the strains/stocks of the two sentinel mice PRL houses.
What is the CD-1 and homozygote Nude?
100
This is the process when newly arrived sentinels are housed with previous quarter sentinels.
What is bridging?
100
These are the four steps for whole blood collection in the mouse and rat.
What is: Euthanize one sentinel per box via CO2 Ear tag the previous quarter’s sentinel Exsanguinate into K2EDTA microtainer tubes. Label each tube with: Room Number AWMS lot # box#
100
This is the number of sentinels per rack side
What is two?
100
This is the type of document that you can reference for specific procedures in regards to the rodent health surveillance program.
What is the SOP (911.07)?
200
This is the gender of our sentinel population including rats, cotton rats, mice and guina pigs.
What is female?
200
This is placed on the AWMS cage card to reflect the active quarter
What is a colored sticker illustrating the appropriate quarter.
200
These are the four steps for preparing a live animal (mouse/rat) for shipment.
What is Remove one mouse/rat per each sentinel box per room. House in transport cage with bedding, hydrogel and food. Tape the lid/staple Label the lid/box with the room number
200
This is the number of sentinels needed to monitor three rack sides
Whar are six sentinel animals (3 boxes)
200
These are the three strains/stocks of animals that are bridged at PRL.
What are the CD-1 mouse, Homozygote nude mouse and Sprague Dawley rat?
300
This is the method in which our sentinel animals are exposed
What is dirty bedding transfer?
300
This is the method in which previous quarter's sentinels are identified prior to bridging.
What is ear tagging?
300
There is no blood collection for this species of sentinel?
What is the guinea pig?
300
This is the age in which cotton rat sentinels are received at.
What is 8-10 weeks?
300
For this species we primarily use colony animals throughout the entire year for sentinel testing.
What is the guinea pig?
400
This USDA covered animal is maintained in-house for two quarters.
What is the cotton rat?
400
This is the receptacle for AWMS cage cards post bridging.
What is the disposition box?
400
This species must be survival bled (via subclavia, saphenous vein, jugular vein, cranial vena cava)
What is the cotton rat?
400
This is the quarter in which Innovive lids should be saved
What is the first quarter (quarter A)?
400
These three specific stocks/strains are NOT sent out for live animal shipments.
What are the guina pig, cotton rat and homozygote Nude.
500
This non-USDA covered animal is maintained for two quarters in rooms with Innovive caging.
What is the Homozygote Nu/Nu mouse?
500
This is the flag that is used to identify sentinels of different quarters that have been housed together.
What is the "bridged sentinels" yellow flag?
500
This is the difference between the guinea pig quarterly PRIA and the annual PRIA
What is the pelt swab?
500
This is the maximum number of sentinels you may have in a box during the bridging period
What is three?
500
This method is used to test for C. bovis in rooms with Tecniplast caging?
What is PRIA - plenum swabs?
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