Denaturation
B-mercaptoethanol is a ___ agent that acts to break the _______ bonds
reducing; disulfide
What is the cross-linking agent during the formation of PAG
bis-acrylamide
What is the pH of the Tris-glycine-chloride buffer?
8.3
SDS-Page is able to separate ______ to _______ sized molecules
Medium; Large
What does SDS stands for
Sodium dodecyl sulfate
Give at least 2 noncovalent bonds that are broken down by the action of SDS
hydrogen bonds, hydrophobic interactions, ionic bonds
The polyacrylamide gel must be ___ because we don’t want our proteins to change or react with it chemically
inactive/unreactive
Which among the components of the Tris-glycine-chloride buffer would form the leading edge of the moving ion front?
Cl-
This is the most common DNA stain which has been suspected to cause genetic defects
Ethidium bromide
SDS PAGE is a technique that separates ________ based on their _______
protein; molecular weight
Its function is to increases the density of the sample
glycerol
What are the 2 gels that we need for SDS-Page and their corresponding pH value?
stacking - 6.8; resolving/separating - 8.8
What is the correct arrangement of the components of the electrophoresis buffer and the Protein-SDS complex when run through the separating gel?
Protein SDS complex, Glycine, Cl-
The breakdown of which toxic component of SDS-Page can affect the nervous system?
Polyacrylamide
One of the 3 key components that contains large amount of glycine critical to separation of proteins
Electrophoresis Buffer
Why is there a need for proteins to obtain a nedgative charge before going through electrophoresis?
so that they are able to be dragged down by the positive electric field found at the bottom of the SDS-PAGE set-up
These oxygen radicals are formed from the reaction of two catalysts namely _____ and _______
Ammonium Persulfate (APS) and Tetramethylethylenediamine (TEMED)
Why do larger proteins tend to move slower than smaller proteins despite having a greater net negative charge?
The higher the molecular weight of the protein, the greater the resistance, and the lesser the velocity
SDS-PAGE is used along with which staining technique to determine the presence of specific proteins in a mixture of proteins?
Western blot
What is the rate of migration for small proteins and why
Small proteins migrate faster due to less resistance during electrophoresis