Semester 1 Workflows
This specific tool compares unknown DNA sequences to a reference database through the National Center for Bioinformatics.
What is BLAST?
This adrenaline junkie insists that if you just spin things fast enough, everything will sort itself out
What is a centrifuge?
In this process, each step typically uses the same dilution factor.
What is a serial dilution?
The overall shape of a double stranded DNA molecule
What is a double helix?
An enzyme critical to PCR found in the hot springs of yellowstone
What is Taq Polymerase?
An instrument to determine the concentration and purity of a DNA sample
What is a nanodrop
A hypersensitive perfectionist who refuses to work properly if they aren't able to recalibrate at the start of each day.
What is an analytical balance?
Failing to apply this concept correctly can result in concentrations being off by factors of 1000.
What is unit conversions?
A DNA strand is comprised of these repeating molecules
What is a nucleotide?
An enzyme able to connect fragments of double stranded DNA
What is Ligase
The process of converting the sequence for GFP to the sequence for YFP
What is Site directed mutagenesis?
A routine-obsessed overachiever who cycles through extreme mood swings to make copies of something very small..... again and again and again.
What is a thermocycler?
This constant (6.022 × 10²³) relates moles to the number of molecules.
What is avogadro's number?
DNA is read in this direction
What is 5' to 3'
An enzyme used to remove unwanted RNA during DNA extraction
What is RNAse
The reagent used to protect competent cells during a deep freeze at -80 C
What is glycerol?
This intense enforcer believes that nothing questionable should survive the combination of heat and pressure.
What is an autoclave?
This type of math is used when adjusting reagent volumes for different sample sizes.
What is a proportion?
The reason DNA is negatively charged
What is the phosphate group
Two variables used to optimize restriction digest reactions
What are time and temperature?
The number of base pairs attached to code for a his-tag
What is 18?
A quiet perfectionist who trusts only eye-level judgments.
What is a graduated cylinder?
This unit of measurement is useful when a solution has many different chemicals at varying concentrations
What is 'X' ?
This nitrogenous base is converted to Uracil during transcription
What is thyamine?
Specific sites where Cas 9 enzymes know to cut
What is a PAM sequence?
Protospacer Adjacent Motif