DNA is:
a. Positively charged
b. Negatively charged
c. Neutrally charged
b. Negatively charged
100
Recombinant DNA (rDNA):
a. Naturally found in the nucleus
b. Naturally found in mithochondia
c. Naturally found in bacteria
d. Artificially made in the lab
d. Artificially made in the lab
100
What are the three basic steps of PCR?
a. Denature, anneal, & strand displacement
b. Denature, anneal & extension
c. Strand displacement, synthesis & release
d. Reverse-transcription, anneal & extend
b. Denature, anneal & extension
100
In DNA, complementary nucleotides are joined together by what?
a. Covalent bonds
b. Hydrogen bonds
c. Electrostatic forces
d. Physical attraction
b. Hydrogen bonds
200
If your micropipette is set at 080, how much volume you are about to dispense?
It is impossible to give the right answer without knowing the maximum amount of volume that the pipette in use is able to dispense
200
Which part of the DNA carries the genetic information:
a. Sugar-phosphate backbone
b. Base sequence
c. Phosphate
b. Base sequence
200
A plasmid is:
a. A clone
b. A cell
c. A bacterium
d. A circular piece of DNA in the bacterial cell
d. A circular piece of DNA in the bacterial cell
200
RNA is typically more stable than DNA
a. True
b. False
c. True only at room temperature
d. It depends by the buffer used to elute the RNA
b. False
200
In what phase of mitosis the chromatin has the highest degree of condensation?
a. Anaphase
b. Metaphase
c. Promethaphase
d. Telophase
b. Metaphase
300
The second stop of a micropipette is used to...
Full expel the liquid
300
What is the correct base pairing:
a. Purin-purin
b. Purin-pyrimidin
c. C-G
c. C-G
300
A restriction site:
a. Encodes for an enzyme that cut DNA
b. An enzyme that restricts the growth bacteria
c. A stretch of DNA that is recognized and cleaved(cut) by a restriction enzyme
d. A random sequence
c. A stretch of DNA that is recognized and cleaved (cut) by a restriction enzyme
300
In RT-PCR, RNA is copied into complementary DNA (cDNA) by:
a. Reverse transcriptase
b. Taq DNA polymerase
c. RNA polymerase II
d. Methyltransferase
a. Reverse transcriptase
300
What cellular process would you expect to immediately disrupt if you added a drug that distroys mthe mitotis spindle?
a. Mitosis
b. Protein production
c. Transcription
d. Fat metabolosm
a. Mitosis
400
How many ng correspond to 1 ug?
1000ng
400
In a chromosome, sister chromatids are joined together by what?
a. Centromere
b. Centriole
c. Chromosome
d. Cytoplasm
a. Centromere
400
In what part of the vector the insert DNA is introduced to create a recombinant DNA molecule?
a. Promotor
b. Shine-Dalgarno
c. Randomly
d. Multiple Cloning Site (MCS)
d. Multiple Cloning Site (MCS)
400
RT-PCR allows you to:
a. Produce multiple copies of a particular piece of DNA
b. Quantify the expression of a given gene
c. Detect the expression of a particular mRNA
d. Identify aspecific products
c. Detect the expression of a particular mRNA
400
What is the complementary base sequence of the following sequence ATGCCTACT:
a. TCATCCGTA
b. TACGGATGA
c. AGTAGGCAT
b. TACGGATGA
500
On a P-1000 micropipette, if 1000μl reads: 1
0
0
what does 550μl look like?
0
5
5
500
At the end of mitosis, one cell has become how many?
a. 2 genetically different daughter cells
b. 2 genetically identical daughter cells
c. 4 genetically different daughter cells
d. 4 genetically identical daughter cells
b. 2 genetically identical daughter cells
500
What is the natural function of restriction enzymes?
a. Protect bacteria by methylating viral DNA
b. Protect bacteria by cleaving viral DNA
c. Protect bacteria by methylating their own DNA
d. Allow researchers to perform molecular cloning
b. Protect bacteria by cleaving viral DNA
500
Conventional PCR allows you to:
a. Produce multiple copies of a particular piece of DNA
b. Quantify the expression of a given gene
c. Detect the expression of a particular mRNA
d. Identify aspecific products
a. Produce multiple copies of a particular piece of DNA