Free Energy
Glycolysis/CAC
Electron Transport Chain
Fermentation/
Anaerobic Respiration
Miscellaneous
100
What is the equation for ATP Hydrolysis?
ATP + H20 --> ADP + Pi
100
Glycolysis involves the complete oxidation of _______ into ________.
Glucose, Pyruvate
100
Name two electron carriers.
NADH and FADH2
100
How many ATP are produced by ethanol fermentation?
2 ATP
100

How many unexcused absences are you allowed?

3
200
What makes ATP a "high-energy" molecule? A. The ribose B. Three phosphate groups C. Adenine D. Impossible to tell E. ATP is not a high-energy molecule
B
200
What percentage of ATP produced during glycolysis is done via substrate-level phosphorylation A. 0% B. 10% C. 25% D. 50% E. 100%
What is E
200
In humans, what is the final electron acceptor in the ETC?
Oxygen (O2)
200
As you are running a marathon, you feel your muscles begin to burn. What is this burn?
Lactic Acid
200
Typically, what is the average grade in Bio 172?
B-
300
Which of the following is TRUE of the reaction below involving methane: CH4 + O2 --> CO2 + H20 + energy A. Carbon dioxide has more potential energy than CH4 B. Oxygen acts as an electron donor C. H20 has been oxidized D. The overall reaction is endergonic E. CH4 acts as an electron donor
E
300
Which of the following is TRUE about glycolysis? A. Requires oxygen B. Requires energy input C. End products include CO2 D. Occurs in the matrix of the mitochondria E. B and D
What is B
300
You introduced a toxin that prevents interaction between FADH2 and Complex II of the electron transport chain. However, you still see ATP production. How is this possible?
Electrons from NADH2 will be added to complex I. Quinone will then take these molecule to Complex III. Protons will still be pumped into the intermembrane space and ATP will be produced.
300
What is the most important consequence of fermentation?
Production of NAD+ to be used in glycolysis
300

How many courses does the SLC facilitate? (+/- 2 acceptable)

16

400
In the reaction below, which of the following is TRUE: C6H12O6 + 6 O2 --> 6 CO2 + 6 H20 + energy A. C6H12O6 is reduced B. O2 is reduced C. CO2 is added to pyruvate to make citric acid D. H2O is oxidized E. All of the above
B
400
A step in the citric acid cycle is as follows: Succinate + FAD → Fumarate + FADH2 Which statement is TRUE? A. The conversion of succinate to fumarate is providing the necessary free energy to drive the reaction forward. B. The conversion of FAD to FADH2 is providing the necessary free energy to drive the reaction forward. C. Succinate has less potential energy than FADH2. D. Fumarate has more potential energy than succinate. E. None of the above is true.
What is B
400
The electrons carried by NADH and FADH2 can be A. pumped into the intermembrane space B. transferred to ATP synthase C. moved between proteins of the inner mitochondrial membrane D. transported into the mitochondrial matrix E. All of the above
C
400
You are working at a winery and see a worker introducing air to the wine in order to make it grow faster. What is the problem with doing this?
The wine will stop undergoing ethanol fermentation and undergo oxidative phosphorylation - production of O2 - wine will be nearly alcohol free and undrinkable
400

Where is your facilitator from?

Michigan

500
You are working in your lab attempting to measure the energetics of a common fatty acid synthesis reaction. You find that the reaction is endergonic. You decide to couple this reaction to an ATP hydrolysis reaction in order for the reaction to become spontaneous. However, despite confirmation of successful hydrolysis, the fatty acid synthase reaction still does not proceed spontaneously. Why was this unsuccessful?
The fatty acid synthesis reaction must have a deltaG greater than the amount of energy released by ATP hydrolysis
500
The amount of ATP present in the cell can regulate substrate-level phosphorylation that occurs during glycolysis. Phosphofructokinase, a key enzyme in the glycolysis pathway is a target of this regulation. How do high levels of ATP affect phosphofructokinase activity? A. ATP binds to RNA polymerase and results in less transcription of the phosphofructokinase gene. B. ATP binds to RNA polymerase and results in more transcription of the phosphofructokinase gene. C. ATP binds to the active site of phosphofructokinase and decreases the ability of the enzyme to function. D. ATP binds to binds to another protein in the glycolysis pathway that results in activation of a feedback inhibition loop that inactivates phosphofructokinase. E. None of the answers are correct.
E
500
The inner membrane of a mitochondrion has lots of folds, or cristae, giving it lots of surface area for electron transport chains. Suppose the inner membrane were smooth instead. How would this affect energy production in a cell? Explain your answer.
Lower surface area! Fewer ATP molecules would be produced if the inner mitochondrial membrane were smooth. The cristae allow many electron transport chains to work all at once in a mitochondrion. Since the electron transport chain and chemiosmosis depend on the inner mitochondrial membrane, there would be much less space to pump protons across the membrane.
500
Most agricultural societies have come up with ways to ferment the sugars in barley, wheat, rice, corn, or grapes to produce alcoholic beverages. Historians argue that this was an effective way for farmers to preserve the chemical energy in grains and fruits in a form that would not be eaten by rats or spoiled by bacteria and fungi. Why does a great deal of chemical energy remain in the products of fermentation pathways?
The drop in potential energy from glucose to pyruvate is low, therefore pyruvate is a high energy molecule. Since fermentation products are derived from pyruvate, they have a lot of energy. You don’t see this as much in pyruvate that is converted to Acetyl-CoA for the TCA and ETC because much of the energy is dissipated in those reactions.
500
"Fun" Biology Trivia! Which infectious agent listed below causes diseases that are most difficult to cure? A. Bacteria B. Fungus C. Virus D. Parasite E. Prion
E
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