Social Brain
What did the Yamamuro and colleagues find after chemogenetically suppressing the mPFC-pPVT circuit?
A. Increased motor activity
B. Reduced anxiety-related behavior
C. Reduced sociability without major effects on motor or anxiety-related behavior
D. Complete loss of movement
C. Reduced sociability without major effects on motor or anxiety-related behavior
At what age were the mice isolated?
P21-P35
TRUE or FALSE
eDREADD was used to activate neuronal activity.
True
In Yamamuro and colleagues' study, which interneuron subtype became more excitable after juvenile social isolation?
A. PV interneurons
B. Dopaminergic neurons
C. SST interneurons
D. Purkinje cells
C. SST interneurons
Why is the juvenile period important in this study?
A. The brain is fully mature during this stage
B. Social circuits are still developing during this period
C. Mice stop learning social behavior after weaning
D. The pPVT is inactive during development
B. Social circuits are still developing during this period
Which method was used to measure real-time neuronal activity during social interaction?
A. Patch clamp
B. Fiber photometry
C. MRI
D. EEG
B. Fiber photometry
TRUE OR FALSE
Juvenile social isolation reduced the intrinsic excitability of mPFC-pPVT neurons in adulthood.
TRUE
TRUE OR FALSE
Juvenile social isolation mice showed stronger mPFC-pPVT activation during social exposure than group-housed mice.
False.
GH mice = stronger social activation
JSI mice = impaired activation
What was the purpose of performing c-Fos mapping at the beginning of the study?
A. To permanently silence the mPFC-pPVT pathway
B. To identify brain regions activated during social exposure
C. To measure synaptic excitability directly
D. To genetically modify SST interneurons
B. To identify brain regions activated during social exposure
What happened when Yamamuro and Colleagues chemogenetically activated mPFC-pPVT neurons in JSI mice?
A. Sociability decreased further
B. Motor activity stopped
C. Sociability deficits were rescued
D. Anxiety dramatically increased
.
C. Sociability deficits were rescued
Which group of mice showed reduced sociability and impaired mPFC-pPVT circuit activity?
A. GH mice
B. Control mice
C. JSI mice
D. mCherry mice
C. JSI mice
TRUE OR FALSE
Repeated optogenetic stimulation of the mPFC-pPVT pathway produced a sustained rescue effect even after stimulation ended.
TRUE
Shown in Figure 8e-f
Which subcortical region receiving mPFC projections showed increased activation during social exposure?
Posterior paraventricular thalamus (pPVT)
How did the social behavior and mPFC-pPVT circuit activity differ between GH mice and JSI mice?
GH mice showed stronger sociability and greater activation of the mPFC-pPVT pathway during social exposure, while JSI mice showed reduced sociability and impaired circuit activity.
What does CNO do in DREADD experiments?
Activates DREADD receptors to suppress or activate neurons