Lab Instruments/Use
What was the purpose of the incubator in the Secrets of the Rainforest Lab?
The Incubator maintained a warm temperature for bacteria to grow.
What was the purpose of the lysozyme in the Secrets of the Rainforest Lab?
The lysozyme weakens the bacterial cell wall for when it’s in the freezer.
Which step came first in the Secrets of the Rainforest Lab? (selecting bacterial colonies, purification through protein chromatography, bacterial concentration and lysis, growing cells, or removing bacterial debris)
Growing Cells
Which section of the lab report includes the purpose, hypothesis, and background research?
The Introduction Section
What is defined as an idea or proposition that can be tested by observations or experiments?
The hypothesis.
What was the purpose of the shaking incubator in the Secrets of the Rainforest?
The shaking incubator provided a warm temperature and contact with oxygen for the bacteria to grow.
What was the purpose of the equilibration buffer in the Secrets of the Rainforest Lab?
The equilibration buffer creates good conditions in the column for the GFP protein.
Which step came second in the Secrets of the Rainforest Lab? (selecting bacterial colonies, purification through protein chromatography, bacterial concentration and lysis, growing cells, or removing bacterial debris)
Selecting Bacterial Colonies
Which section of the lab report includes a data table containing each trial, a visual graph depicting the results, and a summary of the numerical data?
The results section.
What is defined as the variable whose characteristics are not numerically measurable?
The qualitative variable.
What was the purpose of the UV light in the Secrets of the Rainforest?
The UV light allowed us to see the green color of the protein.
What was the purpose of the binding buffer in the Secrets of the Rainforest Lab?
The binding buffer attaches the GFP protein to the column and removes hydrophilic proteins.
Which step came third in the Secrets of the Rainforest Lab? (selecting bacterial colonies, purification through protein chromatography, bacterial concentration and lysis, growing cells, or removing bacterial debris)
Bacterial concentration and lysis.
What is the purpose of inserting the GFP genes into the bacteria cells?
To create more copies of the gene and thus make more protein.
What is defined as the variable where all data is organized in order?
The ranked variable.
What was the purpose of the centrifuge in the Secrets of the Rainforest Lab?
The centrifuge separated components by weight.
What was the purpose of the wash buffer in the Secrets of the Rainforest Lab?
The wash buffer removes some hydrophobic proteins from the column (not the GFP protein).
Which step came fourth in the Secrets of the Rainforest Lab? (selecting bacterial colonies, purification through protein chromatography, bacterial concentration and lysis, growing cells, or removing bacterial debris)
Purification through protein chromatography.
What will cause bacterial cells to have the same genetic makeup?
Being from the same, single colony.
Which group is the group exposed to the treatment/experiment?
The experimental group.
What was the purpose of the freezer in the Secrets of the Rainforest Lab?
The freezer caused the bacterial cell wall to break because of cytoplasmic expansion.
What was the purpose of the TE (elution) buffer in the Secrets of the Rainforest Lab?
The elution buffer removes the GFP protein from the column.
Which step came last in the Secrets of the Rainforest Lab? (selecting bacterial colonies, purification through protein chromatography, bacterial concentration and lysis, growing cells)
protein chromatography
Should you finish all of your prescribed antibiotics even though you feel better?
Yes because it will increase the chances of killing all of the bacteria causing your illness.
Which is the measure of how dispersed the data is in relation to the mean?
The standard deviation.