Immunohistochemistry
Preferred fixative for IHC
Formalin
Target tissue for Ziehl-Neelsen stain
Acid Fast Bacilli
Cause of Uniformly weak staining of the nuclear membrane and chromatin in H&E
Over-differentiation
Luxol Fast Blue solution
Luxol Fast blye, 95% alcohol, 10% acetic acid
Von Kossa
Calcium-black, everything else- red
Where is biotin derived from
Egg yolks
Stain used to demonstrate H. pylori
Giemsa
Steps in stain which used metallic substitution?
Silver nitrate- Hypo- counterstain
Van Gieson
picric acid and acid fuchsin
Masson Trichrome
Collagen-green, nuclei- black, muscle RBC cytoplasm- red
Labelling system which absorbs UV light and emits visible light
Fluorochrome
Expected result for mycobacterium after staining is complete
red
Colour amyloid stains using a polarizing filter after staining with Congo red
Apple green
Grams crystal violet
Crystal violet, distilled water, sodium bicarbonate
Target tissue in Toludine blue
Mast cells- purple
Labelling system used for ABC method and does it use a direct or indirect staining technique
Enzyme/chromogen
Stain used which gives gram negative bacteria their red colour
Basic fuchsin
What happens if we used chromic acid in PAS?
Over-oxidation and false negatives
Perl's Prussian Blue
HCl and potassium ferrocyanide
Luxol Fast Blue/ Cresyl Echt Violet
myelin-blue, nuclei- purple, nissl- purple
Two naturally occuring enzymes in the body that require "blocking" during IHC
peroxidase and alkaline phophatase
Reagents used in Ziehl-Neelsen stain (hint: there's 3)
Carbol-fuchsin, Methylene blue, acid alcohol
Purpose of polyacid in Masson Trichrome
remove red dye from more permeable collagen
Aldehyde Fuchsin
Basic fuchsin, 70% alcohol, HCl, paraldehyde
Target tissues in Martius Scarlett Blue
RBC and early fibrin – yellow
Muscle and mature fibrin – red
Collagen and old fibrin - blue