Babies "waa, waa"
This blood most closely resembles arterial blood but is a mixture of both venous and arterial blood.
Capillary
What area of the baby’s foot should you never poke and why?
Back of the heel becasue it is very close to the heel bone (<2.33mm)
If a doctor needs to diagnose Diabetes Melitus, which GTT test is usually ordered?
2hour GTT
Why don't ants get sick?
Because they have little antibodies!
Name a infectious organism that would be classified as a Category A Infectious Substance.
Ebola, Monkeypox, Japanese Encephalitis cultures...
Give 2 limitations of microcollections.
1) Many chemistry test are unreliable, (ie K due to hemolysis).
2) Can't use when tests need a lot of blood (ie Coagulation)
3) Safety issues, more risk of exposure
What happens to a baby with un-diagnosed Phenylketonuria?
Permanent brain damage
What does it mean when blood cultures are ordered "x2" ?
2 sets of blood cultures need to be taken from two different sites. Each set consists of an anaerobic and an aerobic bottle.
The absorbent pack should be put between which layers according to the TDG guidelines?
Primary and Secondary layers
Name the layers for packaging under TDG guidelines AND which layers need to be waterproof?
Primary (waterproof), Secondary (waterproof) and Outer Packaging
After using a lancet for a microcollection on a baby, what is the very next step before collecting blood?
What is the substance that builds up in the blood, turning the skin yellow?
Bilirubin
Before attaching a needle onto a syringe there are 2 very important things that must be done with the syringe. These are...?
1) Prime syringe (pull back and forth on plunger)
2) Make sure the plunger is pushed all the way forward in the barrel so no air is in the syringe.
Name the 3 major classifications under TDG for sending specimens.
1) 1A - Category A - UN2814
2) 1B - Category B - UN3373
3) Exempt Patient Specimens
Dangerous goods are classified into 9 categories. Which classes and Division does the laboratory use regularly.
Category 6.2 (Infectious)
Category 9 (Miscellaneous)
What is the minimum volume of blood that you can collect on a microcollection capillary poke using a Chemistry PST (mint green) on a newborn?
400uL
The 2 marked gradations are 400uL and 600uL
How are babies treated if their "bilirubins" become too high?
What must a phlebotomist always remember to do before collecting blood from a baby with a very high hemoglobin?
Ultra-violet light. Turn OFF the UV light before collection a bilirubin test.
EDTA and sodium citrate work as anticoagulants by doing this to the blood
Chelate calcium (remove it from the blood so clotting can't occur)
When sending plasma or serum "aliquots", what needs to be done to tubes before they are sent?
Parafilm the caps wether screw top or push on, so they don't come off because of vibrations during transport.
Name the 3 main hazards connected to working with Dry Ice.
1) Burns skin
2) CO2 asphyxiation
3) Explodes if kept in a contained space
For a microcollection, why do you collect the lavender tube first?
When the lancet first cuts the tissue, clotting factors are activated and platelet clumping begins immediately. There can be no clotting in the lavender tube.
Besides PKU disorder name 2 other disorders that are tested for using the PKU card.
And how many tests are done from a PKU card in BC?
Hypothyroidism, Galactosemia, Cystic Fibrosis, MCAD Med. Chain Acyl CoA dehydrogenase deficiency.
27 tests done from those dried blood blots!
Where would you find Peritoneal fluid, Pericardial fluid, Pleural fluid and CSF
Around the organs (abdominal cavity), around the heart, around the lungs and around the spinal cord and brain.
Some Chemistry samples are being picked up from St. Paul's and are going to VGH via courier. The are packaged in a Tupperware container and then placed in a Rubbermaid cooler.
Is this type of packaging allowed under TDG?
What type of packaging is this?
Yes (only 1A Packaging P620) need to be specific.
1B or IATA P650
You need to send a Category 1A specimen to Winnipeg Manitoba from the BCCDC.
List the documents and plans that need to be sent with such a specimen. There are 4 items.
1) Shipper's Declaration (Red Thatched borders used for 1A)
2) Itemized List of Contents (how many and how much specimen)
3) ERAP (Emergency Response Assistance Plan)
4) Air waybill (carrier flying to Manitoba)