PPE
What does PPE stand for?
Personal Protective Equipment.
True or false: You are allowed to eat and drink in the lab.
False.
What is the difference between disinfection and sterilization?
Disinfection uses chemicals such as ethanol or bleach to remove MOST microorganisms, while sterilization is the destruction of ALL microorganisms (including spores) using heat.
What does SDS stand for?
What is the purpose of a micropipette?
To measure and transfer very small amounts of liquid, i.e., less than 1 mL.
Name the laboratory equipment that rapidly rotates in order to apply centrifugal force to its contents, thereby allowing the separation of fluids and other molecules according to density.
Centrifuge.
True or False: horseplay is encouraged during lab / while conducting experiments.
What is the acronym for the government agency that regulates workplace safety?
OSHA.
What concentration of bleach is appropriate when cleaning surfaces in lab?
10%.
What are the four colors in the NFPA diamond, and which hazards are they associated with?
1. Red = flammability
2. Yellow = stability/reactivity
3. Blue = health hazards
4. White = special hazards
True or false: all micropipettes use the same size tips.
False.
What is measured by a spectrophotometer?
The amount of light that is transmitted or absorbed through a sample.
Which PPE can be used to protect your feet?
Closed-toed shoes.
What is the proper way to mix water with an acid?
Add the acid to the water.
What concentration of ethanol or isopropanol can be used when cleaning surfaces in the lab?
70%.
In which section of an SDS would you find details about the chemical's appearance, odor, pH, and other physical and chemical characteristics?
Section 9: Physical and Chemical Properties:
What is the lowest setting on a P-10 micropipette?
1.0 microliters.
Which type of microscope uses florescent dyes to generate complex three-dimensional images of tissues and cells?
Confocal microscope.
True or False: Contact lenses may be worn in place of safety goggles.
False.
When may you dump solid waste or broken glass down the drain?
Never.
How does UV light disinfect surfaces?
By breaking down certain chemical bonds, especially nucleic acids, causing microorganisms to be unable to multiply and/or die.
Who is responsible for creating/providing the SDS for a particular chemical?
The manufacturer.
Name two micropipettes that can transfer 80 microliters.
P100 and P200.
What happens to the absorbance of a sample as the concentration of the sample increases?
As the concentration increases the absorbance also increases (because the molecules do the absorbing).
What should you do immediately after removing protective latex gloves?
Hand hygiene, i.e., wash your hands with soap and water or use hand sanitizer.
Which substance may be used to neutralize a spilled acid?
Sodium bicarbonate.
Name three items that can be sterilized in an autoclave.
Surgical Instruments
Glassware
Centrifuge Tubes
Pipette Tips
Chemical Solutions
Animal Food and Bedding
Which section of the SDS contains recommendations on the appropriate response to spills, leaks, or releases, including containment and cleanup practices?
Section 6: Accidental Release Measures
What do you call a micropipette that moves many units of liquid at the same time?
A multichannel pipette.
Which instrument come in both vertical and horizontal configurations?
Gel electrophoresis apparatus, i.e., vertical for proteins, horizontal for DNA.
This biosafety level involves the highest level of containment, necessary for handling agents with a high potential for aerosol transmission and severe or lethal diseases.
BSL-4
(1) The food can become contaminated by lab materials, causing you to ingest harmful substances.
(2) Lab materials can become contaminated by food particles, affecting the results of an experiment.
In a laboratory setting, which "cleaning" tool effectively eliminates spores?
An autoclave.
What two general types of information does SDS contain?
(1) Potential hazards, e.g. health, fire, reactivity and environmental, etc.
(2) How to work safely with a chemical product
Which stop should you go to if you want to take fluid into a micropipette?
The first stop.
What is the advantage of using a water bath over a Bunsen burner or other direct heat source?
It is easier to maintain a constant temperature when using a water bath.