Agars
What does MAC agar differentiate between?
Lactose fermenters and non-fermenters
Which antigen is the flagellar antigen?
H antigen
What makes Klebsiella pneumoniae so mucoid?
polysaccharide capsule
Which enteric is the only oxidase positive organism?
Plesiomonas
Enterics are gram ________.
negative
What color is normal flora on HE agar?
orange
In the LPS, what causes toxicity?
Lipid A
Which organism swarms on BAP?
Proteus
Name one of the three enterics that would be negative for motility for a SIM test.
Shigella, Klebsiella, Yersinia
Which enteric is a curved gram negative rod?
Vibrio
How can Shigella and Salmonella be differentiated on HE agar?
Which virulence factor antigen is part of the LPS?
O (somatic) antigen
What color are most enteric colonies on BAP?
dull and gray
Which Shigella sp. is positive for ONPG and ODC?
Shigella sonnei
Do enterics have visible spores on gram stains?
No, they're non-spore forming
What temperature is a CIN plate incubated at?
Room temperature
Which antigen covers the O antigen if an organism possesses it?
K (envelope) antigen
Which enteric has brick red colonies on BAP?
Serratia
What does an ONPG disc test test for? (Bonus: what color is positive?)
slow lactose fermentation through detection of beta-galactosidase enzyme (yellow)
Which enteric has "gull wing" formation?
Campylobacter jejuni
Which enteric is MAC with sorbitol meant to isolate and identify?
E. coli O157:H7
What is the name for the type of diarrhea caused by enterotoxigenic Escherichia coli?
Traveler's diarrhea
When beta hemolysis is observed on BAP, which enteric must be ruled out?
Escherichia coli
Which Klebsiella species is indole positive?
Klebsiella oxytoca
Which primary pathogen enteric exhibits bipolar (safety pin) staining?
Yersinia sp.