Streak Isolation 1
Streak Isolation 2
Gram Staining 1
Gram Staining 2
Miscellaneous
100
What is the streak isolation method?
This microbiological technique is used to separate individual bacterial cells on an agar plate to obtain pure colonies.
100
This tool is commonly sterilized by flame before each streaking step to prevent contamination.
What is an inoculating loop?
100
A differential staining technique, named after the physician Hans C. J. Gram, used to characterize the cellular structure of Gram-positive bacteria (which stains a purple color) and Gram-negative bacteria (which stains a pink color)
What is gram staining?
100
This is the primary stain used first in the Gram staining procedure.
This is the primary stain used first in the Gram staining procedure.
100
What are illnesses acquired in a hospital or facility, appearing 48+ hours after admission.
What are nosocomial infections, or healthcare-associated infections (HAIs)
200
What is done in order to dilute the bacterial sample and reduce cell density?
flaming the loop between quadrants during streaking
200
This pattern of streaking divides the agar plate into sections to progressively thin out bacterial growth.
What is the quadrant streak method?
200
These bacteria retain the crystal violet stain and appear purple.
What are Gram-positive bacteria?
200
During this step, alcohol or acetone is used to remove stain from certain bacteria.
What is the decolorization step?
200
What is this?
The Enterotube is a rapid, self-contained, 12-compartment plastic system designed to identify Gram-negative bacteria, particularly Enterobacteriaceae, within 18–24 hours.
300
What are isolated colonies?
These are the visible, well-separated growths on an agar plate that arise from a single bacterial cell or group of identical cells.
300
How are bacteria named?
The first letter of the genus is always capitalized, and both names are always italicized OR underlined. Many times the genus is abbreviated by its first letter and the species is written out. For example: Escherichia coli can also be written as E. coli or E. coli.
300
After decolorization, this stain is applied to visualize Gram-negative bacteria as pink.
What is safranin?
300
These bacteria retain the crystal violet stain and appear purple.
What are Gram-positive bacteria?
300
a serious immune response that can cause serious health problems
What is septic shock?
400
What is a pressure chamber used to sterilize equipment, media, and waste by applying high-pressure saturated steam, typically at 
(
) for 15–60 minutes.
What is an autoclave?
400
The practice and/or process of preventing contamination.
What is aseptic technique?
400
What are the 3 shapes bacteria can be categorized by?
What are cocci, bacilli, and spirilla
400
This structural difference in bacterial cell walls explains why Gram-positive bacteria retain the crystal violet stain.
What is a thick peptidoglycan layer?
400
How can septic shock cause death?
What is a drop in blood pressure?
500
What is a common food used for culturing bacteria. It provides the glucose, amino acids, vitamins, and nutrients many bacteria need to live.
What is Tryptic soy agar (TSA)?
500
What is a rapid form of asexual reproduction used by prokaryotes (bacteria, archaea) and some single-celled eukaryotes to produce two genetically identical daughter cells.
What is binary fission?
500
Why are gram negative bacteria more pathogenic?
Gram negative bacteria have a lipopolysaccharide coating, which typically makes them more pathogenic.
500
iodine - this reagent forms a complex with crystal violet, helping it bind more strongly to bacterial cell walls.
What is the mordant?
500
Why are gram negative bacteria more pathogenic?
Gram negative bacteria have a lipopolysaccharide coating, which typically makes them more pathogenic.