Lab Math
Beer's Law equation
What is A = C?
What is A=E*b*C?
What is A= C*L*K?
Act that mandated that the more complex the testing, the higher the standards of personnel performing them
What is CLIA?
The absorption/transmission of light through a colored solution providing a means of measuring concentration of analyte
What is Spectrophotometry?
Type of pipette that requires remaining fluid to be pushed out
What is a 'To Contain' pipette?
When two consecutive test results from the same specimen have exactly the same value
What is accuracy?
A solution of 6.0mg/dL uric acid has an A=0.25 and the A of an unknown sample is 0.70. What is uric acid concentration of the sample?
C= 16.8mg/dL
Standard that urges personnel to wear PPE and use universal precautions.
What is the Blood Borne Pathogens Standard?
The amount of light passing through a sample; inversely proportional to absorbance.
What is transmittance?
Bonus: What is the equation that relates absorbance to percent transmission?
"Wet" Chemistry is to ___________ as "Dry" Chemistry is to ____________.
Continuous flow analyzer
Discrete analyzer
Type of error that consistently shifts values in one direction
What is a systematic error?
Equation used to convert F to C.
What is C=5/9 x (F-32)?
Fire involving flammable or combustible liquids.
Class B Fire
Measures light scattered by particles in a solution to determine amount of analyte; light detector is placed at a specific angle (90, 70, or 37) from solution.
What is nephelometry?
Analyzers that sequentially perform a test on each of a group of specimens.
What is Batch testing?
Set of rules for interpreting control results; described in terms of standard deviation.
What are Westgard rules?
You have 60ml of pure HCl. How much diluent do you need to add in order to turn it into a 20% (v/v) solution?
240ml diluent
What the acronym RACE stands for.
Rescue.
Alarm.
Contain.
Extinguish.
Sample containing analyte is dissolved in “mobile phase” which carries it through a “stationary phase“
What is chromatography?
Type of detection method used primarily with dry slide technology.
What is reflectance photometry?
Physiologic factors that affect test results.
What is an uncontrollable variable?
How much 2N sulfuric acid (H2SO4) is needed to neutralize 180ml of 2.5N NaOH?
225ml
The PPE required to be used when doing labs in this class.
Wear lab coat. Wear gloves. Keep long hair tied back. Wear closed toed shoes.
The equation used to convert the change in voltage, or potential, to the analyte concentration.
What is the Nernst equation?
The phase that is the most error-prone part of specimen handling.
What is the preanalytical phase?
Most common tube drawn for chemistry tests.
Clot activator SSTs (tiger tops)