CRISPR
Hydrogels
mRNA Vaccines
About Tyler
100
What nuclease cuts the DNA in the CRISPR system we discussed?

Cas9

100

Hydrogels absorb large amounts of what molecule?

Water

100

What does the mRNA in COVID vaccines encode for?

Spike Protein

100

What university do I attend?

MIT

200

What type of RNA is complementary to the DNA of interest and is used to “program” the CRISPR system enzyme?

gRNA or crRNA

200

What type of molecule is a hydrogel composed of?

(Hydrophilic) Polymers 
200

What is a molecule that stimulates the immune system called?

Antigen

200

Where is my hometown?

Hawaii

300

What type of organism did CRISPR-Cas originate in?

Bacteria

300

Name one use of hydrogels that we discussed.

Agriculture, diapers, contact lenses, artifical organs

300

Name one advantage of mRNA vaccines over traditional vaccines.

Can be quickly produced/tested, cheaper to produce, lack of interaction with host DNA
300

What is my major (area of study) at my university?

Biological Engineering

400

What type of repair mechanism is error-prone but quick?

Non Homologous End Joining

400

What is the mesh size of a hydrogel?

Distance between cross-links between polymers

400

What type of cell produces antibodies in humans?

B cells
400

What pet did I use to have (that I mentioned in my fun fact)?

Octopus

500

What type of repair is needed to create specific mutations?

Homology Directed Repair

500

Name one way to make firmer hydrogels using sodium alginate and CaCl2.

Higher concentration of CaCl2, Longer soaking time

500

What component of lipid nano particles allows mRNA to enter the cell?

Ionizable lipids

500

How old am I?

20

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