Section of DNA chromatin wrapped around 8 histone proteins
nucleosome
This protein is needed to created an mRNA transcript
RNA polymerase
Enzyme responsible fo relieving supercoiling tension upstream of replication fork
Gyrase/Topoisomerase
The ribosome reads the mRNA from the _' end to the _' end
5' to 3'
Adapter molecules that read the mRNA and carry the amino acid.
What are tRNAs
Why is continuous synthesis on both strand of DNA not possible?
DNA polymerase III can only add nucleotides in the 5' to 3' directions
Which organelle helps polypeptides finish folding and enable their final conformation?
Golgi body/apparatus/complex
What sections of DNA are used for DNA profiling?
Tandem repeat sequences
Permanently supercoiled DNA not available for gene expression
heterochromatin
What is the benefit of alternative splicing to create different mature mRNAs
Multiple proteins can be produced by a single gene
Multiple ribosomes attached to a single mRNA
polysome
What type of bonds/interactions are responsible for holding tertiary and quaternary protein structures together
Hydrogen bonds
Ionic Bonds
Dissulfide bridges
Hydrophobic/hydrophilic interactions
What are the steps of Sanger's Sequencing method
Fluorescent dye markers are attached to dideoxyribonucleic acids so that the base present when replication stops can be identified. From this the base on the parent strand is deduced.
DNA replication is carried out with dideoxyribonucleic acid mixed in with normal deoxyribonucleic acid.
A range of new strands of differing lengths are produced.
The length of strand and the terminal base are identified by sequencing machines
Process that inhibits transcription by bind the DNA more tightly to the histone making it less accessible
Acetylation
Complexes of proteins and small RNAs that carry out RNA splicing.
Spliceosome
Required in the termination of translation in order for the ribosome subunits to detach from the mRNA
Release factor
What is the study of epigenetics trying to assess?
What possible impacts can environmental or social factors have on humans