Media
The most common media used in microbiology and can detect hemolysis.
What is sheep blood agar?
These organisms appear as pairs and chains in gram stains.
What are Streptococcus spp.?
A group of gram-negative rods that ferment glucose and are found in the gastrointestinal tract.
What are Enterobacteriaceae?
3% H2O2 is used for this test.
What is catalase?
Worldwide disease most commonly found in developing countries and causes watery diarrhea.
What is traveler's diarrhea (ETEC)?
An enriched media which has lysed red blood cells and facilitates growth of fastidious organisms.
What is chocolate agar?
This commonly isolated organism can be beta-hemolytic and coagulase positive.
What is Staphylococcus aureus?
A fastidious gram-negative rod that requires NAD and hemin for growth.
What is H. influenzae?
This test should be performed on alpha hemolytic colonies.
What is the optochin disc?
Nonfermenting organism that is oxidase positive and associated with cystic fibrosis patients.
What is Pseudomonas aeruginosa?
This media contains bile salts and is differential for gram-negative organisms.
What is MacConkey agar?
This organism is most often beta-hemolytic, catalase negative and hippurate positive.
What is S. agalactiae (Group B strep)?
This organism often appears mucoid on culture, is lactose positive and is commonly isolated in urine cultures.
What is Klebsiella pneumoniae?
This common test can help distinguish between E. coli and K. pneumoniae.
What is indole?
This catalase negative, gram-positive cocci is known to have a caramel or butterscotch odor.
What is Streptococcus anginosus?
This selective enriched media isolates gram-positives from swarming organisms.
What is CNA or PEA?
A coagulase negative and novobiocin resistant isolate most commonly seen in urine cultures.
What is S. saprophyticus?
Name a pathogenic Enterobacteriaceae.
What is Salmonella, Shigella or Yersinia?
This test should be performed on non-hemolytic streptococci.
What is bile esculin?
This gram-negative diplococci can cause otitis media in children and is part of the normal flora in the upper respiratory tract.
What is Moraxella catarrhalis?
This media is used to isolate Neisseria spp. from sites with normal flora.
What is Thayer Martin or Martin Lewis agar?
A beta-hemolytic, catalase negative, PYR positive isolate that is bacitracin sensitive.
What is S. pyogenes (Group A strep)?
A curved gram-negative rod that is oxidase positive, can grow in 0% NaCl and causes rice-water stools.
What is Vibrio cholera?
This test should be done on all H. influenzae isolates.
What is beta-lactamase?
This toxin type can be produced by both gram-positive and gram-negative organisms.
What are exotoxins?