Clone Wars
Organ-ization
The Operon Model
Fertilization: The Urchin Way
Cleavage Complications
100

This is the Central Dogma

What is Gene Expression: Replication, Transcription, and Translation

100

Explain the etymology of the suffix -genesis, as in Organogenesis

Meaning origin, creation, generation, or pertaining to origins.

100

This is the main purpose of operons

To regulate transcription

100

These two words mean "having half the number of chromosomes" and "having homologous copies of each chromosome," respectively

What are haploid and diploid

100
A series of rapid cell divisions during early development that occur in the absence of cell growth

What is Cleavage

200

This cloning experiment supported the differential expression hypothesis from Steward's Carrot experiment AND introduced the understanding of how cell differentiation restricts genetic potential over time.

What is Gurdon's Tadpole experiment

200

This is the stage where we begin to first see cellular differentiation and distinct cell layers

What is Gastrulation

200

This group of life uses operons to regulate their DNA

What are Prokaryotes


200

This fact about the process of sea urchin spawning makes them a great tool to study fertilization. 

What is external fertilization

200

This is a fluid-filled cavity surrounded by a hollow ball of cells

What is a blastocoel
300

This is the difference between cloning plant experiments and cloning animal experiments?  

What is nuclear transplantation, or somatic cell nuclear transfer
300

This determines the dorsal-ventral axis

The entry of the sperm, which causes cortical rotation, and forms the gray crescent

300

In this type of regulation, the regulatory protein is a repressor and turns transcription off

What is negative regulation 

300

Why are twins not a case of polyspermy?

Identical and Fraternal twins are both cases where the egg to sperm ratio is 1:1.
300

Uneven distribution of these into blastomeres during early cleavages sets up the cells for cell differentiation

What are Cytoplasmic Determinants

400

Identify the difference between Embroyonic Stem cells and Adult Stem cells, and define each term!

Adult Stem cells are pluripotent (aka, can differentiate into a limited number of cell types)

Embroyonic Stem cells are totipotent (aka, can differentiate into any cell)

400

This is an observed phenomenon where a single genotype may produce multiple phenotypes depending on environmental conditions

It is also an example of eukaryotic gene regulation

What is developmental plasticity

400

Explain two processes through which eukaryotes regulate transcription 

Acetylation - chromatin made accessible for transcription

Methylation - chromatin condensed and not available for transcription

400

This is the slow block to polyspermy

What are sodium ions entering into the fertilized egg cell causing depolarization across the egg cell membrane
400

This fact separates chicken embryos development from frog and sea urchin development 

NO cleavage through the yolk, and the formation of a blastodisc on top of the yolk. 
500

Describe the process by which Dolly the sheep was cloned

1. Mammary cell taken from donor and "dedifferentiated"

2. Egg cell taken from donor and "enucleated"

3. Dedifferentiated nucleus transplanted into enucleated egg cell and implanted into surrogate mother

500

A mutation in these would cause development to proceed in a way where morphology of appendages changes drastically. Identify what and why

What are Hox Genes, because they are master developmental genes that directly control the identity of body parts

500

This is the difference between negative regulation of the TRP operon and of the LAC operon

What is: Binding of the signal molecule.

Binding of the signal molecule in the TRP operon activates the repressor to turn the operon off 

Binding of the signal molecule in the LAC operon inactivates the repressor to turn the operon on

500

Explain the purpose of the acrosome, and where it is found

The acrosome, found in the sperm, penetrates the egg's jelly coat and releases hydrolytic enzymes to digest it. The acrosomal process has bindin proteins that bind to species specific receptor proteins on the egg's plasma membrane, triggering the slow block to polyspermy
500

This is what happens when there is a moderate amount of yolk present in a fertilized egg cell (think: frog)

What is asynchronous cell division, formation of an animal and vegetal pole, and slower cleavage through the yolky hemisphere

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