Where's Tide-to-Go When You Need It
A stain used to delineate or differentiate between two major groups of bacteria based on the structure of the cell envelope
What is Gram Stain?
A living structure that is metabolically inactive and resistant to heat, chemical treatments, and radiation.
What are endospores?
Bacteria whose morphology is characterized by a rod shape
What is Bacillus?
Who is credited as the first scientist to view and describe microorganisms using a microscope
Who is Antony van Leeuwenhoek?
Five basic techniques to manipulate, grow, examine and characterize microorganisms in the laboratory
What are the Five I's?
Inoculation, Incubation, Isolation, Inspection, and Identification
A necessary step to kill and secure the specimen to the slide while also preserving cellular components in a natural state with minimal distortion
What is heat fixation or heat fixing?
Organisms that are too small to be seen with the unaided eye
Microorganisms
What are the three most common morphological shapes?
Bacillus, Cocci, and Vibrio (Spiral)
Scientist who used swan neck flask in an experiment that disproved the theory of spontaneous generation
Who is Louis Pasteur?
A requirement of any instrument used for sampling and inoculation
What is free of microbes (sterile)?
Genus of organisms that require acid-fast staining
What are Mycobacterium?
True or False
All bacteria and archaea are microorganisms, but only some eukaryotes are microorganisms
True
A patient has a serious respiratory infection. A sputum sample yielded a bacterium that did not have any peptidoglygan. You hypothesize that the identify of this microbe could possible be a ________.
What is Mycoplasma?
The three Domains
What is Eukarya, Archaea, and Bacteria?
Media is classified according to three properties
What are physical state, chemical composition, and function type/purpose?
You need to determine if a pure culture of bacteria is Gram + or Gram -, but you spilled your only solution of crystal violet so you cannot do a Gram Stain. You decide to try a lysozyme treatment on a sample of each culture and them examine the samples under the microscope. The expected result is...
The Gram + cells will lyse and appear as debris
The Gram - will be unaffected
What are magnification, resolution, and contrast?
I am a kind of cocci in chains
What is Streptococcus?
A German physician that is credited as one of the founders of bacteriology and microbiology. Studied anthrax, TB, and cholera.
Who is Robert Koch?
Three techniques for growing microbes in lab on plates
What are streak plate method, pour plate / loop dilution method, and spread plate method?
What are two types of special stains mentioned in your textbook?
What is a capsule stain? What is a flagella stain?
The magnification of the objective lens on a Bright-field microscope
What is 4X, 10X, 40X, and 100X?
You've just isolated a new bacterium in pure culture, and you culture it on a general purpose medium where its cells have a coccobacillus morphology. When you examine the cells after culturing on a variety of differential media, you find that in some cases, the cells appear as coocobacillus, but in others, they can be filamentous, cocci, or club-shaped. The best explanation for this observation is that...
Are the bacterium pleomorphic?
Father of Taxonomy. His system for naming, ranking, and classifying organisms is still in wide use today
Who is Carl Linnaeus, also known as Carl von Linné or Carolus Linnaeus?
The Gram stain, acid-fast stain, and endospore stain have this is common
What are differential stains?