What technique is used to concentrate light rays into the objective lens, thus increasing the resolving power of the lens?
(Labs 1 & 2) (Objective: Be able to use correct microscope technique, including the use of the oil immersion lens.)
oil immersion
100
Where can microbes be found in a school environment?
(Lab 3) (Objective: Understand the importance of microbes.)
everywhere
100
What is the name of the process that involves using water and dry heat to attach a specimen to a slide? (Lab 6) (Obj. Identify steps of the gram staining process.)
heat fixing
100
This technique was used throughout multiple labs and involves killing microbes using dry heat from a Bunsen burner...
(Lab 4) (Objective: Identify aseptic techniques.)
flaming
100
An cluster of microbe growth that can be observed on a Petri dish is called... (Lab 5) (Objective: Identify bacteria from growth on a nutrient agar plate.)
a colony
200
What type of microscope would be best suited for viewing living specimens?
(Lab 1) (Objective: Compare and contrast various types of light and electron microscopes and name situations in which each is best used.)
compound light microscope or "bright-field" microscope
200
The substance that is extracted from seaweed and used to grow a wide range of microbes within a sterile Petri dish is... (Lab 5 and Selective Media Lab) (Objective: Distinguish different types of media used to grow bacteria.)
nutrient agar
200
A mordant is a substance that improves the resolution of n object when viewed under a microscope. What mordant was used when conducting the gram staining procedure on the bacteria collected from the school environment? (Lab 7) (Objective: Identify steps of gram staining.)
iodine
200
The goal of creating and maintaining an environment that is free of microbes is known as... (Lab 4) (Objective: Identify aseptic techniques.)
aseptic technique
200
This type of media suppresses or prevents the growth of one group of microbes while permitting the growth of another group. (Selective Media Lab) (Objective: Contrast selective and differential medias.)
selective
300
The total degree of magnification is found by multiplying what two values?
(Labs 1 & 2) (Objective: Be able to use correct microscope technique, including the use of the oil immersion lens.)
the magnification of the ocular lens by the magnification of the objective lens
300
In order to promote microbe growth by increasing the temperature of the environment, Petri dishes are placed inside of what device? (Labs 3, 4, 5, & Selective Media Lab) (Objective: Distinguish different types of media used to grow bacteria.)
an incubator
300
The gram staining process is conducted to determine what information about a bacteria based on the structure of its cell wall? (Lab 7) (Obj. Identify basic techniques for identifying bacteria.)
whether the bacteria is gram positive or gram negative
300
What lab instrument was used to transfer microbes? (Lab 4) (Obj. Identify aseptic techniques.)
an inoculating loop or sterile cotton swab
300
The technique of spreading bacteria over the surface of a sterile Petri dish containing nutrient agar in order to create isolated colonies is called what? (Lab 5) (Objective: Identify bacteria from growth on a nutrient agar plate.)
streak plate method
400
Many specimens that are viewed under a microscope are smaller than 1 millimeter. Because of this, measurements are often expressed using what unit?
(Lab 2) (Objective: Understand that microbiology includes a diversity of microorganisms requires the regular use of the microscope.)
microns
400
Is a wet or dry environment more suitable for microbe growth? (Lab 3) (Objective: Distinguish different types of media used to grow bacteria.)
wet environment
400
What is the name of the red counterstain used in the gram stain process? (Lab 7) (Obj. Identify steps of the gram staining process.)
safranin
400
A student examines two separate test tubes that both contain the same broth. The broth in test tube A is clear while the broth in test tube B is cloudy with solid objects floating near the surface. Which test tube was successfully inoculated with a microbe? (Lab 4) (Obj. Identify aseptic techniques.)
test tube B
400
What type of media can help to identify different groups of microbes by means of changing color? (Selective Media Lab) (Objective: Contrast selective and differential medias.)
differential
500
When using a compound light microscope with the high power objective lens in place, what is the total magnification of the object being viewed?
(Labs 1 & 2) (Objective: Be able to use correct microscope technique, including the use of the oil immersion lens.)
400 X
500
A student is viewing bacteria under a microscope. The bacteria has a pink color and is arranged in clusters of rods. What type of bacteria is the student viewing? (Lab 3) (Objective: Identify bacteria from growth on nutrient agar plate.)
gram negative staphylobacilli
500
What causes the dyes used in the gram staining process to stick to the surface of the bacteria? (Lab 7) (Objective: Describe why the stain sticks to the cell wall of the bacteria.)
the magnetic attraction between the positively charged dyes and the negatively charged bacteria
500
Aseptic technique is a common practice in what type of facility? (Lab 4) (Objective: Identify aseptic techniques.)
hospitals, clinics, nursing homes, etc.
500
What is the name of the specific agar used to inhibit the growth of gram-positive bacteria? (Selective Media Lab) (Objective: Distinguish different types of media used to grow bacteria.)