TSI- Why oh Why?
This tribe of Enterobacterales is positive for phenylalanine deaminase.
Proteae tribe
Proteus, Providencia, Morganella
Endemic Cholera
Vibrio cholerae (O1)
Non-fermenters fail to ferment this substance.
Carbohydrates/glucose
This organism causes plague- usually from the bite of a flea.
What is Yersinia pestis?
The indole test detects the presence of this enzyme.
This is the only Enterobacterales that is oxidase positive.
Plesiomonas
Undercooked hamburger ingestion, lettuce outbreaks, HUS
E. coli O157:H7
Name the two pigments produced by Pseudomonas aeruginosa.
Pyocyanin and Pyoverdin
Duodenal ulcers, gastric carcinoma.
What is Helicobacter pylori?
The Enterobacteriaceae all share the following characteristics. Name the one that is not listed here:
Reduce Nitrate to Nitrite
Oxidase negative (except one species)
Gram negative Rods
Facultative anaerobes, 18-24 hours growth
Grow on MacConkey
What is Glucose fermentation!
Gamma hemolytic
TSI: A/A, H2S NEG
Motile: 25 degrees only
Indole: Weak +
Oxidase: Neg
Urea: POS
Yersinia enterocolitica
The temperature at which Campylobacter spp. must be incubated.
42 degrees C
This test will distinguish between Acinetobacter and Stenotrophomonas.
DNAse or Citrate (Steno is pos for both)
DOUBLE JEOPARDY!
Name the two most common pneumonia causing pathogens in Cystic Fibrosis patients?
The H antigen is what bacterial structure.
Flagella!
K=Capsule
O=Outer membrane
Ox/Indole: NEG/NEG
TSI: K/A, H2S POS
LIA: NEG/NEG/H2S POS
UREA: NEG
Motile
Salmonella
This organ harbors Salmonella in carrier patients if left untreated.
Gallbladder
Newborn meningitis, caused by a nonfermenting GNR
Elizabethkingia meningoseptica!
Freshwater wound after traumatic ski accident?
Aeromonas
What is the urea breath test/urea biopsy test?
The one biochemical test needed to differentiate between Morganella and Providencia.
Citrate (Pos in Providencia)
A beta hemolytic, oxidase positive, indole positive lactose fermenter was isolated from a patient with gastroenteritis. This organism is the most likely ID.
Aeromonas
Many patients who have a tracheostomy tube in the ICU become colonized with THIS non-fermenting GNR.
Acinetobacter spp.
Appendicitis-like syndrome in adults
What is Yersinia enterocolitica?
When serotyping Salmonella, we need to remove THIS antigen by heating the organism in order to serotype the O antigen.
What is the Vi antigen?